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DAPK3/ZIPK Antibody - BSA Free

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Western Blot: DAPK3/ZIPK Antibody [NBP1-76962] - Analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody [NBP1-76962] - Staining of ZIPK in Jurkat cells with ZIPK antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody [NBP1-76962] - Staining of ZIPK in Jurkat cells with ZIPK antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody - BSA Free [NBP1-76962] - Immunofluorescence of DAPK3/ZIPK in Jurkat cells with DAPK3/ZIPK antibody at 10 u/mL.
Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody - BSA Free [NBP1-76962] - Immunofluorescence of DAPK3/ZIPK in Jurkat cells with DAPK3/ZIPK antibody at 10 u/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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DAPK3/ZIPK Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase. The immunogen is located within amino acids 270 - 320 of ZIPK. Amino Acid Squence: RRRNVRGEDSGRKPERRRLK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DAPK3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Western Blot 1 ug/ml
Application Notes
An approximate 40 kDa band can be detected in Western Blot, which represents the pro-enzyme of DNase II.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DAPK3/ZIPK Peptide (NBP1-76962PEP)
Publications
Read Publication using NBP1-76962.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for DAPK3/ZIPK Antibody - BSA Free

  • DAP kinase 3
  • DAPK3
  • DAP-like kinase
  • death-associated protein kinase 3
  • Dlk
  • EC 2.7.11
  • EC 2.7.11.1
  • ZIP kinase isoform
  • ZIP
  • ZIPK
  • ZIPKFLJ36473
  • ZIP-kinase

Background

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase . ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAPK3/ZIPK Antibody (NBP1-76962)(1)

We have publications tested in 1 application: IHC-P.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DAPK3/ZIPK Antibody (NBP1-76962) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DAPK3
Uniprot