Home » MLK3 » MLK3 Antibodies » MLK3 Antibody
Novus Biologicals products are now on bio-techne.com

MLK3 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4559) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MLK3 at approximately 93 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human MLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4559) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4559-SP
Novus Biologicals offers 100% Guarantee

MLK3 Antibody Summary

Immunogen
E. coli-derived recombinant human MLK3
Ala85-Gly207
Accession # Q16584
Specificity
Detects endogenous human MLK3 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
MAP3K11
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MLK3 Antibody

  • EC 2.7.11
  • EC 2.7.11.25
  • MAP3K11
  • MEKK11
  • mitogen-activated protein kinase kinase kinase 11
  • Mixed lineage kinase 3
  • MLK3
  • MLK3MLK-3
  • PTK1
  • PTK1protein-tyrosine kinase PTK1
  • RHOE
  • SH3 domain-containing proline-rich kinase
  • SPRK
  • SPRKMGC17114
  • Src-homology 3 domain-containing proline-rich kinase

Background

Mixed-lineage kinases (MLKs) function as mitogen-activated protein kinase kinase kinases (MAP3Ks), activating the MAPK signaling cascades. MLK3, also known as MAP3K11 and PTK1, encodes a protein of 847 amino acids (aa) that shares an unusual leucine zipper‑basic motif found in other MLKs. Small interfering RNA experiments have indicated that MLK3 is required for both mitogen and cytokine activation of JNK1, p38 alpha , and ERK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

JNK1 Antibody
(228601) [Unconjugate
MAB17761
Western Blot JNK1 Antibody (228601) [Unconjugated]
Western Blot JNK1 Antibody (228601) [Unconjugated]
Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC, WB
c-jun [p Ser63]
Antibody (1018Y) [Unc ...
MAB8930
Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
MAP3K13 Antibody
NBP1-81272
Western Blot MAP3K13 Antibody
Immunohistochemistry-Paraffin MAP3K13 Antibody
Immunohistochemistry-Paraffin MAP3K13 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
JNK3 Antibody
(29E10)
NBP3-26149
Immunohistochemistry JNK3 Antibody (29E10)
Western Blot JNK3 Antibody (29E10)
Immunocytochemistry/ Immunofluorescence JNK3 Antibody (29E10)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, WB
MKK4/MEK4 Antibody
(OTI8A8)
NBP1-47839
Flow Cytometry MKK4/MEK4 Antibody (OTI8A8)
Immunocytochemistry/ Immunofluorescence MKK4/MEK4 Antibody (OTI8A8)
Immunocytochemistry/ Immunofluorescence MKK4/MEK4 Antibody (OTI8A8)
(1 Review)
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(50 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
MKK7/MEK7 Antibody
(4E5) - BSA Free
NBP2-37542
ELISA MKK7/MEK7 Antibody (4E5) - BSA Free
Western Blot MKK7/MEK7 Antibody (4E5) - BSA Free
Immunocytochemistry/ Immunofluorescence MKK7/MEK7 Antibody (4E5) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
p38 alpha Antibody
[Unconjugated]
AF8691
Simple Western p38 alpha Antibody [Unconjugated]
Western Blot p38 alpha Antibody [Unconjugated]
Immunohistochemistry p38 alpha Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
CDC42 Antibody
(JJ086-04)
NBP2-67895
Flow Cytometry CDC42 Antibody (JJ086-04)
Immunohistochemistry-Paraffin CDC42 Antibody (JJ086-04)
Immunohistochemistry-Paraffin CDC42 Antibody (JJ086-04)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
MAP3K12 Antibody
NBP2-17218
Western Blot MAP3K12 Antibody
Western Blot MAP3K12 Antibody
Immunohistochemistry-Paraffin MAP3K12 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
Glutamate Receptor
6 Antibody (4B4L9)
NBP3-16867
Western Blot Glutamate Receptor 6 Antibody (4B4L9)
Western Blot Glutamate Receptor 6 Antibody (4B4L9)
Immunohistochemistry Glutamate Receptor 6 Antibody (4B4L9)
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
Crk Antibody
(3G11E8) - BSA Free
NBP2-37568
ELISA Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(822 Publications)
Species: Hu
Applications: BA
POLDIP2 Antibody
NBP1-68874
Western Blot POLDIP2 Antibody
Western Blot POLDIP2 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
AHSA1 Antibody
(1A2-A8)
H00010598-M01
Western Blot AHSA1 Antibody (1A2-A8)
Sandwich ELISA AHSA1 Antibody (1A2-A8)
(6 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Flow Cytometry GRAP2 Antibody (OTI1G2)
Flow Cytometry GRAP2 Antibody (OTI1G2)
Western Blot GRAP2 Antibody (OTI1G2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AIMP2 Antibody
NBP1-81575
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(63 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
MLK3 Antibody
AF4559
MLK3 Antibody
Species: Hu
Applications: WB

Publications for MLK3 Antibody (AF4559) (0)

There are no publications for MLK3 Antibody (AF4559).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MLK3 Antibody (AF4559) (0)

There are no reviews for MLK3 Antibody (AF4559). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MLK3 Antibody (AF4559) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MLK3 Products

Blogs on MLK3

There are no specific blogs for MLK3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Donkey anti-Sheep IgG Secondary Antibody [HRP]
HAF016
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MLK3 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol MAP3K11
Uniprot