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MAD2L1 Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-563AF350
Alexa Fluor 405
NB100-563AF405
Alexa Fluor 488
NB100-563AF488
Alexa Fluor 532
NB100-563AF532
Alexa Fluor 594
NB100-563AF594
Alexa Fluor 647
NB100-563AF647
Alexa Fluor 700
NB100-563AF700
Alexa Fluor 750
NB100-563AF750
Allophycocyanin
NB100-563APC
Biotin
NB100-563B
DyLight 350
NB100-563UV
DyLight 405
NB100-563V
DyLight 488
NB100-563G
DyLight 550
NB100-563R
DyLight 594
NB100-563DL594
DyLight 650
NB100-563C
DyLight 680
NB100-563FR
DyLight 755
NB100-563IR
FITC
NB100-563F
HRP
NB100-563H
Janelia Fluor 525
NB100-563JF525
Janelia Fluor 549
NB100-563JF549
Janelia Fluor 646
NB100-563JF646
Janelia Fluor 669
NB100-563JF669
PE
NB100-563PE
PerCP
NB100-563PCP
Unconjugated
NB100-563
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MAD2L1 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAD2L1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MAD2L1 Antibody [CoraFluor™ 1]

  • HSMAD2
  • MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
  • MAD2 mitotic arrest deficient-like 1 (yeast)
  • MAD2L1
  • MAD2-like protein 1
  • MAD2mitotic arrest deficient, yeast, homolog-like 1
  • Mitotic arrest deficient 2-like protein 1
  • mitotic spindle assembly checkpoint protein MAD2A

Background

The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAD2L1