![Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048] - Sections of the tibial articular cartilage of 12-week-old normal Mig-6-flox (C) and Mig-6-flox; Prx1Cre conditional knockout (Mig-6-cko) knees (D) immunostained with antibodies against superficial zone protein (SZP, C, D). In normal articular cartilage, immunostaining is localized to cells mainly in the superficial zone (arrows in C) while in Mig-6-cko articular cartilage, immunostaining is dramatically increased and positive cells are located not only in the superficial zone but also in deeper zones (arrows in D). Scale Bar = 50 um. Image collected and cropped by CiteAb from the following publication (arthritis-research.biomedcentral.com/articles/10.1186/ar4233), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lubricin-PRG4-Antibody-Immunohistochemistry-NBP1-19048-img0002.jpg "Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048] - Sections of the tibial articular cartilage of 12-week-old normal Mig-6-flox (C) and Mig-6-flox; Prx1Cre conditional knockout (Mig-6-cko) knees (D) immunostained with antibodies against superficial zone protein (SZP, C, D). In normal articular cartilage, immunostaining is localized to cells mainly in the superficial zone (arrows in C) while in Mig-6-cko articular cartilage, immunostaining is dramatically increased and positive cells are located not only in the superficial zone but also in deeper zones (arrows in D). Scale Bar = 50 um. Image collected and cropped by CiteAb from the following publication (arthritis-research.biomedcentral.com/articles/10.1186/ar4233), licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Synthetic peptide derived from N terminal domain of PRG4 protein |
| Specificity | Reacts specifically with human 151 kDa protein |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PRG4 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P, IHC reactivity reported in scientific literature (PMID: 23705804), (23083677). |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Antiserum |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Unpurified |
| Reconstitution Instructions | Lyophilized powder, reconstituted in distilled water. |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048] - Sections of six-week old tibial articular cartilage of normal Mig-6-flox (M) and Mig-6-cko (N) joints immunostained with antibodies against superficial zone protein (SZP, M, N). Enhanced marker expression by cells in the superficial and middle zones is evident in the six-week-old Mig-6-cko articular cartilage compared to control Mig-6-flox articular cartilage (arrows). Scale bar = 100 nm. Image collected and cropped by CiteAb from the following publication (arthritis-research.biomedcentral.com/articles/10.1186/ar4233), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lubricin-PRG4-Antibody-Immunohistochemistry-NBP1-19048-img0001.jpg "Immunohistochemistry: Lubricin/PRG4 Antibody [NBP1-19048] - Sections of six-week old tibial articular cartilage of normal Mig-6-flox (M) and Mig-6-cko (N) joints immunostained with antibodies against superficial zone protein (SZP, M, N). Enhanced marker expression by cells in the superficial and middle zones is evident in the six-week-old Mig-6-cko articular cartilage compared to control Mig-6-flox articular cartilage (arrows). Scale bar = 100 nm. Image collected and cropped by CiteAb from the following publication (arthritis-research.biomedcentral.com/articles/10.1186/ar4233), licensed under a CC-BY license.")
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![Simple Western MIF Antibody (12302) [Unconjugated]](https://images.novusbio.com/images2/MIF_MAB289_Simple_Western_18975.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")