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LMP2/PSMB9 Antibody (792520)

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line treated (+) with 100 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse ...read more
LMP2/PSMB9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cells stimulated with Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours using Mouse Anti-Human LMP2/PSMB9 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
792520
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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LMP2/PSMB9 Antibody (792520) Summary

Immunogen
E. coli-derived recombinant human LMP2/PSMB9
Thr21-Glu219
Accession # P28065
Specificity
Detects human LMP2/PSMB9 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human PSMB6 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PSMB9
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LMP2/PSMB9 Antibody (792520)

  • beta1i
  • LMP2
  • LMP2MGC70470
  • Low molecular mass protein 2
  • Macropain chain 7
  • Multicatalytic endopeptidase complex chain 7
  • proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalpeptidase 2)
  • proteasome catalytic subunit 1i
  • Proteasome chain 7
  • proteasome subunit beta 6i
  • proteasome subunit beta type-9
  • Proteasome subunit beta-1i
  • proteasome-related gene 2
  • PSMB6iproteasome (prosome, macropain) subunit, beta type, 9 (large multifunctionalprotease 2)
  • PSMB9
  • Really interesting new gene 12 protein
  • RING12
  • RING12EC 3.4.25.1

Background

LMP2 (large multifunctional peptidase 2), also called PSMB9 (proteasome subunit beta 9) or PSMB6i, is a 22 kDa broad-specificity aminopeptidase of the T1B family that is a component of the 20S immunoproteasome core (a7b7b7a7) of the 26S proteasome particle. Induction of LMP2 by IFN-g, IRF-1, TNF-a, CD40L, or heat shock in immune-responsive cells or cancer cells promotes replacement of proteasome subunit PSMB6 by LMP2 and processing of peptides for presentation by MHC I. After auto-cleavage between amino acids (aa) 20-21, human LMP2 (aa 21-219) shares 91% aa sequence identity with mouse and rat LMP2. Alternate splicing creates a short form, LMP2.S, which lacks aa 4-13 within the propeptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PSMB9
Uniprot