![Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Analysis in human prostate and skeletal muscle tissues using NBP1-90286 antibody. Corresponding Filamin B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90286-img0028.jpg "Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Analysis in human prostate and skeletal muscle tissues using NBP1-90286 antibody. Corresponding Filamin B RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: KEPGEYAVHIMCDDEDIKDSPYMAFIHPATGGYNPDLVRAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGEGQRIDIQMKNRMDGTYACSYTPVKAIKHTIAVVWG |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FLNB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FLNB |
![Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human colon, placenta, prostate and skeletal muscle using Anti-Filamin B antibody NBP1-90286 (A) shows similar protein distribution across tissues to independent antibody NBP1-90285 (B).](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90286-img0027.jpg "Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human colon, placenta, prostate and skeletal muscle using Anti-Filamin B antibody NBP1-90286 (A) shows similar protein distribution across tissues to independent antibody NBP1-90285 (B).")
![Western Blot: Filamin B Antibody [NBP1-90286] - Analysis in human cell lines A-549 and HEK293 using anti-FLNB antibody. Corresponding FLNB RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Western-Blot-NBP1-90286-img0017.jpg "Western Blot: Filamin B Antibody [NBP1-90286] - Analysis in human cell lines A-549 and HEK293 using anti-FLNB antibody. Corresponding FLNB RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.")
![Immunocytochemistry/Immunofluorescence: Filamin B Antibody [NBP1-90286] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90286-img0015.jpg "Immunocytochemistry/Immunofluorescence: Filamin B Antibody [NBP1-90286] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human skeletal muscle shows low expression in myocytes as expected.](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90286-img0032.jpg "Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human skeletal muscle shows low expression in myocytes as expected.")
![Western Blot: Filamin B Antibody [NBP1-90286] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Western-Blot-NBP1-90286-img0007.jpg "Western Blot: Filamin B Antibody [NBP1-90286] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90286-img0029.jpg "Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human prostate shows strong membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human colon shows strong membranous postivity in glandular cells.](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90286-img0030.jpg "Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human colon shows strong membranous postivity in glandular cells.")
![Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Filamin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-90286-img0031.jpg "Immunohistochemistry-Paraffin: Filamin B Antibody [NBP1-90286] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.")
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")