LIF Antibody (9824) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells (17).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Development, IHC-Fr, Neutralization, Western Blot.

Showing Publications 1 - 8 of 8.

Publications using MAB250	Applications	Species
F Kano, N Hashimoto, Y Liu, L Xia, T Nishihara, W Oki, K Kawarabaya, N Mizusawa, K Aota, T Sakai, M Azuma, H Hibi, T Iwasaki, T Iwamoto, N Horimai, A Yamamoto Therapeutic benefits of factors derived from stem cells from human exfoliated deciduous teeth for radiation-induced mouse xerostomia Scientific Reports, 2023-02-15;13(1):2706. 2/15/2023 [PMID: 36792628]
Ning Ke, Lin Chen, Qing Liu, Haibo Xiong, Xinke Chen, Xiyuan Zhou Downregulation of miR-211-5p Promotes Carboplatin Resistance in Human Retinoblastoma Y79 Cells by Affecting the GDNF–LIF Interaction Frontiers in Oncology 3/2/2022 [PMID: 35311096]
Xin Chen, Bryan S. Hausman, Guangbin Luo, Guang Zhou, Shunichi Murakami, Janet Rubin et al. Protein Kinase Inhibitor gamma Reciprocally Regulates Osteoblast and Adipocyte Differentiation by Downregulating Leukemia Inhibitory Factor Stem Cells 2013-12-01 [PMID: 23963683]
Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS. J. Biol. Chem., 2011-07-22;286(39):34271-85. 2011-07-22 [PMID: 21784845] (Western Blot, Human)	Western Blot	Human
Eriko Simamura, Hiroki Shimada, Nobuaki Higashi, Maimi Uchishiba, Hiroki Otani, Toshihisa Hatta Maternal Leukemia Inhibitory Factor (LIF) Promotes Fetal Neurogenesis via a LIF-ACTH-LIF Signaling Relay Pathway Endocrinology 2010-04-01 [PMID: 20160138]
Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells. Blood, 2007-09-11;110(13):4319-30. 2007-09-11 [PMID: 17848619] (Neutralization, Human)	Neutralization	Human
Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries. Mol. Hum. Reprod., 2004-03-25;10(5):313-9. 2004-03-25 [PMID: 15044601] (IHC-Fr, Human)	IHC-Fr	Human
Hess S, 2019, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes. 7046, 2001-10-01;44(10):2392-401. 2001-10-01 [PMID: 11665982] (ELISA Development, Human)	ELISA Development	Human

Array MAB250

• LIF Antibodies
• LIF ELISA Kit
• LIF Lysate
• LIF Proteins
• LIF Proteins and Enzymes
• LIF ELISAs