Home » Lgr5/GPR49 » Lgr5/GPR49 Antibodies » Lgr5/GPR49 Antibody (CL13567)
Novus Biologicals products are now on bio-techne.com

Lgr5/GPR49 Antibody (CL13567)

 Submit a Review

Images

 
(
Enlarge)
Staining of human skeletal muscle shows moderate membranous positivity in myocytes.
Staining of human skeletal muscle shows moderate membranous positivity in myocytes.
Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Western blot analysis in human cell line SH-SY5Y.
Staining of human ovarian cancer (dysgerminoma) shows strong membranous and cytoplasmic positivity in tumor cells.
Staining of human cerebral cortex shows moderate membranous positivity in a subset of neurons.
Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong cytoplasmic positivity in tumor cells.
Orthogonal Strategies: Analysis in human fallopian tube and tonsil tissues using NBP3-24570 antibody. Corresponding LGR5 RNA-seq data are presented for the same tissues.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clone
CL13567
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Lgr5/GPR49 Antibody (CL13567) Summary

Immunogen
This antibody was designed to target the recombinant protein Lgr5/GPR49, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (91%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LGR5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Reactivity Notes

Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Lgr5/GPR49 Antibody (CL13567)

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CD133 Antibody -
BSA Free
NB120-16518
Immunohistochemistry-Paraffin CD133 Antibody - BSA Free
Western Blot CD133 Antibody - BSA Free
Chromatin Immunoprecipitation CD133 Antibody - BSA Free
(40 Publications)
Species: Hu, Mu, Po, Rt
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
CD44 Antibody
(2C5) [Unconjugated] ...
BBA10
Simple Western CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
Western Blot CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
Immunohistochemistry CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
(5 Reviews)
(49 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
BMI-1 Antibody
(LLBmi1-1) - BSA Free
NBP1-96140
Flow Cytometry BMI-1 Antibody (LLBmi1-1) - BSA Free
Flow Cytometry BMI-1 Antibody (LLBmi1-1) - BSA Free
Immunocytochemistry/ Immunofluorescence BMI-1 Antibody (LLBmi1-1) - BSA Free
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: ChIP, Flow-IC, Flow, ICC/IF, IP, WB
Lgr6 Antibody
(918719) [Unconjugate
MAB8458
Flow Cytometry Lgr6 Antibody (918719) [Unconjugated]
Immunocytochemistry Lgr6 Antibody (918719) [Unconjugated]
(1 Publication)
Species: Hu
Applications: CyTOF-ready, Flow, ICC
Musashi-1 Antibody
[Unconjugated]
AF2628
Western Blot Musashi-1 Antibody [Unconjugated]
Immunohistochemistry Musashi-1 Antibody [Unconjugated]
Immunocytochemistry Musashi-1 Antibody [Unconjugated]
(7 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IHC, WB
DCLK1 Antibody -
BSA Free
NBP1-77127
Western Blot DCLK1 Antibody - BSA Free
Immunohistochemistry-Paraffin DCLK1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence DCLK1 Antibody - BSA Free
(1 Publication)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Lgr4/GPR48
Antibody (852229) [Un ...
MAB7750
Immunocytochemistry Lgr4/GPR48 Antibody (852229) [Unconjugated]
Flow Cytometry Lgr4/GPR48 Antibody (852229) [Unconjugated]
Blockade of Receptor-ligand Interaction Lgr4/GPR48 Antibody (852229) [Unconjugated]
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC
Oct-3/4 Antibody
[Unconjugated]
AF1759
Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]
Immunocytochemistry Oct-3/4 Antibody [Unconjugated]
Simple Western Oct-3/4 Antibody [Unconjugated]
(73 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
beta-Catenin
Antibody [Unconjugate
AF1329
Western Blot beta-Catenin Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]
Simple Western beta-Catenin Antibody [Unconjugated]
(2 Reviews)
(31 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
ALCAM/CD166
Antibody [Unconjugate
AF1172
Immunohistochemistry ALCAM/CD166 Antibody [Unconjugated]
Immunocytochemistry ALCAM/CD166 Antibody [Unconjugated]
Western Blot ALCAM/CD166 Antibody [Unconjugated]
(2 Reviews)
(22 Publications)
Species: Ca, Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
APC Antibody - BSA
Free
NB100-91662
Immunocytochemistry/ Immunofluorescence APC Antibody - BSA Free
Flow Cytometry APC Antibody - BSA Free
Immunohistochemistry-Paraffin APC Antibody - BSA Free
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IL-13 [Biotin]
DY413
N/A IL-13 [Biotin]
(146 Publications)
Species: Mu
Applications: ELISA
Doublecortin
Antibody
NBP1-72042
Western Blot Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
Immunocytochemistry/ Immunofluorescence Doublecortin Antibody
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
R-Spondin 1
[Unconjugated]
7150-RS/CF
Bioactivity R-Spondin 1 [Unconjugated]
(9 Publications)
Species: Mu
Applications: BA
CD24 Antibody
(ML5) - BSA Free
NB100-77903
Flow Cytometry CD24 Antibody (ML5) - BSA Free
Flow Cytometry CD24 Antibody (ML5) - BSA Free
Immunocytochemistry/ Immunofluorescence CD24 Antibody (ML5) - BSA Free
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-Fr
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)
(24 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Lgr5/GPR49
Antibody (CL13567)
NBP3-24570
Lgr5/GPR49 Antibody (CL13567)
Species: Hu, Mu
Applications: WB, IHC

Publications for Lgr5/GPR49 Antibody (NBP3-24570) (0)

There are no publications for Lgr5/GPR49 Antibody (NBP3-24570).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Lgr5/GPR49 Antibody (NBP3-24570) (0)

There are no reviews for Lgr5/GPR49 Antibody (NBP3-24570). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lgr5/GPR49 Antibody (NBP3-24570) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Lgr5/GPR49 Products

Research Areas for Lgr5/GPR49 Antibody (NBP3-24570)

Find related products by research area.

Blogs on Lgr5/GPR49.

Check out the latest blog posts on Lgr5/GPR49.
Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lgr5/GPR49 Antibody (CL13567) and receive a gift card or discount.


Bioinformatics

Gene Symbol LGR5