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LAP (TGF-beta 1) Antibody (7F6) - BSA Free

Images

 
Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)
Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.
Flow Cytometry: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Flow cytometric analysis of A549 cells using LAP (TGF-beta 1) mouse mAb (green) and negative control (red).
Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis of human stomach tissue (A) and human small intestine tissue (B) using TGF beta 1 antibody (NBP2-22114) at 2 ug/ml.
Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Human tonsil. Image from verified customer review.
Immunohistochemistry-Paraffin: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using LAP (TGF-beta 1) mouse mAb with DAB staining.
ELISA: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
7F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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LAP (TGF-beta 1) Antibody (7F6) - BSA Free Summary

Immunogen
Purified recombinant fragment of human TGF beta 1 (amino acids 62-197) expressed in E. coli. [UniProt# P01137]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TGFB1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry reported in scientific literature (PMID 30147399)
  • Immunohistochemistry-Paraffin reported by customer review
  • Western Blot 1:500-1:2000
Theoretical MW
44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 3.7
using
NBP2-22114 in the following applications:

Publications
Read Publications using
NBP2-22114 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26851347). Rat reactivity reported in scientific literature (PMID: 30147399).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for LAP (TGF-beta 1) Antibody (7F6) - BSA Free

  • CED
  • DPD1
  • LAP (TGFbeta 1)
  • LAP (TGF-beta 1)
  • LAP
  • TGFB
  • TGFB1
  • TGFbeta
  • transforming growth factor beta 1

Background

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, IHC

Publications for LAP (TGF-beta 1) Antibody (NBP2-22114)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: IF/IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, WB.


Filter By Application
IF/IHC
(3)
IHC-Fr
(2)
IHC-P
(4)
Immunocytochemistry/ Immunofluorescence
(1)
WB
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
Rat
(2)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NBP2-22114 Applications Species
Mousset A, Lecorgne E, Bourget I et al. Neutrophil extracellular traps formed during chemotherapy confer treatment resistance via TGF-? activation Cancer cell 2023-04-10 [PMID: 37037615] (WB, Mouse) WB Mouse
Sholqamy M TGF- beta Paradox and the Progression of Osteoblastoma versus Osteosarcoma. Egyptian Dental Journal 2021-10-01
Lang M, Krump C, Meshcheryakova A et al. Microenvironmental and cell intrinsic factors governing human cDC2 differentiation and monocyte reprogramming Frontiers in Immunology 2023-07-19 [PMID: 37539048] (Immunocytochemistry/ Immunofluorescence) Immunocytochemistry/ Immunofluorescence
Garlapati V, Molitor M, Michna T et al. Targeting myeloid cell coagulation signaling blocks MAP kinase/TGF-?1-driven fibrotic remodeling in ischemic heart failure Journal of Clinical Investigation 2023-02-15 [PMID: 36548062]
Wu X, Shen Q, Chang H et al. Promoted CD4(+) T cell-derived IFN-?/IL-10 by photobiomodulation therapy modulates neurogenesis to ameliorate cognitive deficits in APP/PS1 and 3xTg-AD mice Journal of Neuroinflammation 2022-10-10 [PMID: 36217178]
Mohamed MZ, Abed El Baky MF, Ali ME, Hafez HM Aprepitant exerts anti-fibrotic effect via inhibition of TGF-beta/Smad3 pathway in bleomycin-induced pulmonary fibrosis in rats Environmental toxicology and pharmacology 2022-08-02 [PMID: 35931359] (IHC-P, Rat)

Details:
1:100 IHC-P
IHC-P Rat
Griffin J, Krolikowski J, Kounga K et al. Red Light Mitigates the Deteriorating Placental Extracellular Matrix in Late Onset of Preeclampsia and Improves the Trophoblast Behavior Journal of Pregnancy 2022-04-20 [PMID: 35494491] (IHC-Fr, Human) IHC-Fr Human
Malik MNH, Waqas SFH, Zeitvogel J et al. Congenital deficiency reveals critical role of ISG15 in skin homeostasis The Journal of clinical investigation 2021-11-30 [PMID: 34847081] (IF/IHC, Human) IF/IHC Human
Singh J, Thachil T, Eapen M Et Al. Immunohistochemical investigation of cytokine expression levels as biomarkers in transrectal ultrasound-guided needle biopsy specimens of prostate adenocarcinoma Molecular and Clinical Oncology 2021-07-16 [PMID: 34405051] (IHC-P) IHC-P
Wang J, Lee CJ, Deci MB et al. MiR-101a loaded extracellular nanovesicles as bioactive carriers for cardiac repair Nanomedicine 2020-04-08 [PMID: 32278100] (IF/IHC, IHC-Fr, Mouse) IF/IHC, IHC-Fr Mouse
Show All 17 Publications.

Reviews for LAP (TGF-beta 1) Antibody (NBP2-22114) (3) 3.73

Average Rating: 3.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-22114:
Filter by Applications
WB
(1)
IHC-P
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot LAP (TGF-beta 1) NBP2-22114
Enlarge
2
reviewed by:
Shatovisha Dey
WB Mouse 10/02/2020
View

Summary

ApplicationWestern Blot
Sample TestedMouse Pancreas
SpeciesMouse
Lot384830
  4
reviewed by:
Verified Customer
IHC-P Human 10/28/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman kidney
SpeciesHuman
Lot130520
Immunohistochemistry-Paraffin LAP (TGF-beta 1) NBP2-22114
Enlarge
5
reviewed by:
Hayley Derricott
IHC-P Human 04/04/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
Lot130102

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LAP (TGF-beta 1) Antibody (NBP2-22114). (Showing 1 - 1 of 1 FAQs).

  1. Info regarding this product NBP2-22114SS, does this product cross reacts with Latent TGF-beta 1 also?
    • Based on the immunogen sequence, we predict it would cross react.

Secondary Antibodies

 

Isotype Controls

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Research Areas for LAP (TGF-beta 1) Antibody (NBP2-22114)

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Blogs on LAP (TGF-beta 1)

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Recent Reviews

3.7
5
1
4
1
3
0
2
1
1
0

Shatovisha Dey
10/02/2020
Application: WB
Species: Mouse

 
Verified Customer
10/28/2015
Application: IHC-P
Species: Human

Hayley Derricott
04/04/2014
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol TGFB1
Entrez
Uniprot