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LAD1 Antibody

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Orthogonal Strategies: Western Blot: LAD1 Antibody [NBP1-86126] - Analysis in human cell lines A-431 and HEK293 using Anti-LAD1 antibody. Corresponding LAD1 RNA-seq data are presented for the same cell lines. ...read more
Immunocytochemistry/ Immunofluorescence: LAD1 Antibody [NBP1-86126] - Staining of human cell line MCF7 shows localization to actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126] - Staining of human small intestine shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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LAD1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SPRTISFRMKPKKENSETTLTRSASMKLPDNTVKLGEKLERYHTAIRRSESVKSRGLPCTELFVAPVGVASKRHLFEKELAGQSRAEPASSRKENLRLSGVVTSRLNLWISRTQESGDQDPQEAQKASSATERTQWGQKSDSSL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
LAD1 Protein (NBP1-86126PEP)
Publications
Read Publication using
NBP1-86126 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for LAD1 Antibody

  • LAD
  • lad-1
  • LadA
  • ladinin 1
  • ladinin-1
  • linear IgA bullous dermatosis antigen
  • linear IgA disease antigen homolog
  • Linear IgA disease antigen
  • MGC10355

Background

LAD1 is encoded by this gene may be an anchoring filament that is a component of basement membranes. It may contribute to the stability of the association of the epithelial layers with the underlying mesenchyme. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAD1 Antibody (NBP1-86126)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAD1 Antibody (NBP1-86126) (0)

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Secondary Antibodies

 

Isotype Controls

Additional LAD1 Products

Array NBP1-86126

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Bioinformatics

Gene Symbol LAD1