Lactate Dehydrogenase A/LDHA Antibody - BSA Free

Lactate dehydrogenase (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of NADH and NAD+. Mammalian LDH has five tetrameric isozymes comprising combinations of two subunits (i) A subunit (LDHA or LDHM) predominates in skeletal muscle where it involve in anaerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predominates in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including renal, breast, gastric, nasopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient anaerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several malignancies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertional myoglobinuria and glycogen storage disease type 11 (GSD11).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 9 applications: B/N, ELISA, ICC/IF, IF/IHC, IHC-Fr, IHC-P, S-ELISA, Simple Western, WB.

Showing Publications 1 - 10 of 39. Show All 39 Publications. Collapse Publications.

Publications using NBP1-48336	Applications	Species
Cappellesso F, Orban MP, Shirgaonkar N et al. Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer Nature cancer 2022-12-01 [PMID: 36522548] (WB, Mouse)	WB	Mouse
Nsiah NY, Inman DM. Destabilizing COXIV in M�ller Glia Increases Retinal Glycolysis and Alters Scotopic Electroretinogram Cells 2022-11-24 [PMID: 36497016] (ICC/IF)	ICC/IF
Hu Z, Yu X, Ding R et al. Glycolysis drives STING signaling to facilitate dendritic cell antitumor function The Journal of clinical investigation 2023-02-23 [PMID: 36821379] (WB, Mouse)	WB	Mouse
Ahmad F, White M, Yamamoto K et al. Metabolic and imaging phenotypes associated withRB1loss in castrate resistant prostate cancer bioRxiv 2023-11-17 (WB, Human)	WB	Human
Wicks JC Understanding Beef Quality Development and Different Feeding Regimes Thesis 2023-01-01 (WB, Bovine) Details: 1:30000 dilution	WB	Bovine
Marwarha G, Rosand O, Slagsvold KH, Hoydal MA GSK3 beta Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia International journal of molecular sciences 2022-08-19 [PMID: 36012628] (S-ELISA, Human)	S-ELISA	Human
Pappenhagen N, Yin E, Morgan AB et al. Stretch stress propels glutamine dependency and glycolysis in optic nerve head astrocytes Frontiers in neuroscience 2022-08-05 [PMID: 35992925] (Simple Western, WB, Rat)	Simple Western, WB	Rat
Schwab M, Multhoff G A Low Membrane Hsp70 Expression in Tumor Cells With Impaired Lactate Metabolism Mediates Radiosensitization by NVP-AUY922 Frontiers in oncology [PMID: 35463341] (WB, Mouse)	WB	Mouse
Marwarha G, Rosand o, Scrimgeour N et al. miR-210 Regulates Apoptotic Cell Death during Cellular Hypoxia and Reoxygenation in a Diametrically Opposite Manner Biomedicines 2021-12-25 [PMID: 35052722] (ELISA)	ELISA
Schwab M, Thunborg K, Azimzadeh O et al. Targeting Cancer Metabolism Breaks Radioresistance by Impairing the Stress Response Cancers (Basel) 2021-07-27 [PMID: 34359663] (WB, B/N)	WB, B/N
Shen W, Lee SR, Mathai AE et al. Effect of selectively knocking down key metabolic genes in MUller glia on photoreceptor health Glia 2021-04-09 [PMID: 33835598]
Pinto IF, Soares RRG, MAkinen ME et al. Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production ACS sensors 2021-03-26 [PMID: 33724791]
Osis G, Traylor AM, Black LM et al. Expression of Lactate Dehydrogenase A and B isoforms in the mouse kidney American journal of physiology. Renal physiology 2021-03-15 [PMID: 33719570]
Weiss-Sadan, T, Itzhak, G Et al. Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation. Mol Cell Proteomics 2019-07-01 [PMID: 33451519] (IF/IHC, Human)	IF/IHC	Human
Knowles HJ Distinct roles for the hypoxia-inducible transcription factors HIF-1alpha and HIF-2alpha in human osteoclast formation and function Scientific reports 2020-12-03 [PMID: 33273561] (WB, Human)	WB	Human
Rimmer L Investigating the metabolic changes that accompany skeletal muscle hypertrophy Thesis 2023-01-01 (WB)	WB
Dang DS, Buhler JF, Thornton KJ et al. Myosin heavy chain isoform and metabolic profile differ in beef steaks varying in tenderness Meat Sci. 2020-07-28 [PMID: 32739757] (WB, Bovine)	WB	Bovine
Gizak A, McCubrey JA, Rakus D. Cell-to-cell lactate shuttle operates in heart and is important in age-related heart failure Aging (Albany NY) 2020-02-08 [PMID: 32035422]
Jo YH Hydrocarboxylic acid receptor 1 in BAT regulates glucose uptake in mice fed a high-fat diet PLoS ONE 2020-01-30 [PMID: 31999787] (WB, Mouse)	WB	Mouse
Duda P, Janczara J et al. The Reverse Warburg Effect is Associated with Fbp2-Dependent Hif1 alpha Regulation in Cancer Cells Stimulated by Fibroblasts. Cells 2020-01-14 [PMID: 31947613] (ICC/IF, Mouse)	ICC/IF	Mouse
Dang DS Role of Mitochondria in Postmortem Proteolysis and Meat Tenderness Thesis 2022-01-01 [PMID: 31935569] (WB, Bovine)	WB	Bovine
Apaoblaza A, Gerrard SD, Matarneh SK et al. Muscle from grass- and grain-fed cattle differs energetically Meat Sci. 2019-11-06 [PMID: 31734468] (WB, Bovine)	WB	Bovine
Choi YS, Song JE, Lee JE et al. Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice Mol Brain 2019-11-01 [PMID: 31675964] (WB, Mouse)	WB	Mouse
Boyle J, Patronas NJ, Smirniotopoulos J et al CRH stimulation improves 18F-FDG-PET detection of pituitary adenomas in Cushing's disease. Endocrine. 2019-07-01 [PMID: 31062234] (IHC-P, Human) Details: Citation using the Alexa Fluor 488 version of this antibody.	IHC-P	Human
Rimmer L, Geisbrecht E, Chao M et al. Investigating the metabolic changes that accompany skeletal muscle maturation Physiology 2023-05-01
Xu Q, Li X et al. Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation1. J Anim Sci 2019-02-07 [PMID: 31267132] (WB, Bovine)	WB	Bovine
Kaushik DK, Bhattacharya A, Mirzaei R et al. Enhanced glycolytic metabolism supports transmigration of brain-infiltrating macrophages in multiple sclerosis J. Clin. Invest. 2019-05-21 [PMID: 31112527] (WB, Mouse)	WB	Mouse
Schwab M, Dezfouli A, Khosravi M et al. The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response Research Square 2023-03-20 (WB)	WB
Weiss Sadan, T;Itzhak, G;Kaschani, F;Yu, Z;Mahameed, M;Anaki, A;Ben-Nun, Y;Merquiol, E;Tirosh, B;Kessler, B;Kaiser, M;Blum, G; Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation Mol. Cell Proteomics 2019-04-22 [PMID: 31010818] (WB, Human)	WB	Human
Xu, Q;Li, X;Ma, L;Loor, JJ;Coleman, DN;Jia, H;Liu, G;Xu, C;Wang, Y;Li, X; Adipose tissue proteomic analysis in ketotic or healthy Holstein cows in early lactation J. Anim. Sci. 2019-04-15 [PMID: 30985866] (Bovine)		Bovine
Dodo M, Kitamura H, Shima H et al. Lactate Dehydrogenase C Is Required for the Protein Expression of a Sperm-specific Isoform of Lactate Dehydrogenase A J. Biochem. 2018-12-24 [PMID: 30590713] (WB, Mouse)	WB	Mouse
Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human) Details: Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).		Human
Hulley PA, Bishop T, Vernet A et al. Hypoxia-Inducible Factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2. J. Pathol. 2017-04-18 [PMID: 28418093]
Elgin JM Determining the underlying factors of fresh ham color variation Meat Sci 2017-02-19 [PMID: 28214148]
Takenouchi T, Tsukimoto M, Iwamaru Y et al. Extracellular ATP induces unconventional release of glyceraldehyde-3-phosphate dehydrogenase from microglial cells. Immunol. Lett. 2015-08-12 [PMID: 26277554] (WB, Mouse) Details: Lactate Dehydrogenase A/LDHA Antibody was used for WB on microvesicle and exosome enriched /depleted fractions from LPS-primed MG6 cells (mouse microglial cell line immortalized by a replication-deficient retroviral vector containing human c-myc gene) after ATP stimulation (Fig. 3A).	WB	Mouse
Kim J, Han J, Jang Y et al. High-capacity glycolytic and mitochondrial oxidative metabolisms mediate the growth ability of glioblastoma Int. J. Oncol. 2015-07-21 [PMID: 26202438]
Pastor-Soler NM, Sutton TA, Mang HE et al. Muc1 is protective during kidney ischemia-reperfusion injury Am J Physiol Renal Physiol. 2015-04-28 [PMID: 25925251] (WB, Mouse)	WB	Mouse
Adochite RC, Moshnikova A, Carlin SD et al. Targeting Breast Tumors with pH (Low) Insertion Peptides. Mol. Pharm. 2014-07-08 [PMID: 25004202] (IHC-Fr, Mouse) Details: Lactate Dehydrogenase A antibody used at 1:100 dilution for IHC-Fr application on 4% PFA fixed cryosections of FVB/N-Tg(MMTV-PyVT)634Mul/J transgenic female mice (develops palpable mammary tumors at 12-15 wk of age) as well as tissues from control noncarrier FVB/NJ female mice (Figures 6 and 7). IHC-Fr of LDHA also performed on the cryosections of tumors that were generated by grafting 4T1 mouse mammary tumor cell line on BALB/c mice.	IHC-Fr	Mouse
Show All 39 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	WB	Human	10/09/2023	View Summary Application Western Blot Sample Tested glioblastoma cell lysates Species Human Lot A-4
Enlarge	4 reviewed by: Verified Customer	WB	Human	03/10/2020	View Summary Application Western Blot Sample Tested HepG2 human hepatocellular carcinoma cell line,Huh-7 human hepatoma cell line Species Human Lot b-3 Comments Comments Used it in 1:1000 with milk TBST.
Enlarge	5 reviewed by: Eric England	WB	Other	10/10/2014	View Summary Application Western Blot Sample Tested Whole Skeletal Muscle Lysate Species Other Lot A
Enlarge	4 reviewed by: Eric England	WB	Other	10/01/2014	View Summary Application Western Blot Sample Tested Whole Skeletal Muscle Lysate Species Other Lot A
Enlarge	5 reviewed by: Bryan Tinsley	IHC	Mouse	03/17/2014	View Summary Application Immunohistochemistry Species Mouse

Array NBP1-48336

• Lactate Dehydrogenase A/LDHA Antibodies
• Lactate Dehydrogenase A/LDHA Lysate
• Lactate Dehydrogenase A/LDHA Proteins
• Lactate Dehydrogenase A/LDHA Proteins and Enzyme