![Knockout Validated: Lactate Dehydrogenase B Antibody [NBP2-53421] - Western blot shows lysates of 293 human embryonic kidney parental cell line and LDHB knockout (KO) 293 cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human LDHB Polyclonal Antibody (NBP2-53421) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for LDHB at approximately 35 kDa (as indicated) in the parental 293 cell line, but is not detectable in the knockout 293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Knockout-Validated-NBP2-53421-img0007.jpg "Knockout Validated: Lactate Dehydrogenase B Antibody [NBP2-53421] - Western blot shows lysates of 293 human embryonic kidney parental cell line and LDHB knockout (KO) 293 cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human LDHB Polyclonal Antibody (NBP2-53421) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for LDHB at approximately 35 kDa (as indicated) in the parental 293 cell line, but is not detectable in the knockout 293 cell line. This experiment was conducted under reducing conditions.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Partial recombinant human Lactate Dehydrogenase B protein (amino acids 1-93) [P07195] |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LDHB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publications using NBP2-53421 | Applications | Species |
|---|---|---|
| Schwab M, Dezfouli A, Khosravi M et al. The radiation- and chemo-sensitizing capacity of diclofenac can be predicted by a decreased lactate metabolism and stress response Research Square 2023-03-20 (WB) | WB | |
| Schwab M, Multhoff G A Low Membrane Hsp70 Expression in Tumor Cells With Impaired Lactate Metabolism Mediates Radiosensitization by NVP-AUY922 Frontiers in oncology [PMID: 35463341] (WB, Mouse) | WB | Mouse |
| Kumagai S, Koyama S, Itahashi K Et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments Cancer Cell 2022-01-29 [PMID: 35090594] Details: Citation using the PE version of this antibody. |
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| Lai YC, Hsieh CY, Lu KY Et al. Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study Metabolites 2021-08-06 [PMID: 34436459] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | LDHB |
![Western Blot: Lactate Dehydrogenase B Antibody [NBP2-53421] - Total protein from human HeLa and A431 cells, mouse MEF cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LDHB in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP2-53421-img0003.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP2-53421] - Total protein from human HeLa and A431 cells, mouse MEF cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LDHB in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-LDHB at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53421-img0006.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-LDHB at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP2-53421] - Analysis of a FFPE tissue section of mouse liver using Lactate Dehydrogenase B antibody at 1:400 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated a very specific staining in the hepatocytes.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunohistochemistry-Paraffin-NBP2-53421-img0002.jpg "Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP2-53421] - Analysis of a FFPE tissue section of mouse liver using Lactate Dehydrogenase B antibody at 1:400 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated a very specific staining in the hepatocytes.")
![Flow Cytometry: Lactate Dehydrogenase B Antibody [NBP2-53421] - An intracellular stain was performed on SK-MEL-28 cells with Lactate Dehyrdrogenase B Antibody NBP2-53421F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Flow-Cytometry-NBP2-53421-img0008.jpg "Flow Cytometry: Lactate Dehydrogenase B Antibody [NBP2-53421] - An intracellular stain was performed on SK-MEL-28 cells with Lactate Dehyrdrogenase B Antibody NBP2-53421F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LDHB at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53421-img0001.jpg "Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LDHB at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")