![Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76398_rabbit-monoclonal-kap1-p-ser824-antibody-bl-246-7b5-30320239244.jpg "Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, IP |
| Clone | BL-246-7B5 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155) |
| Modification | p Ser824 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TRIM28 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 2 - 8 C / 1 year from date of receipt |
| Buffer | Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Purity | Antigen Affinity-purified |
![KAP1 [p Ser824] Antibody (BL-246-7B5)](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "KAP1 [p Ser824] Antibody (BL-246-7B5)")
Secondary Antibodies |
Isotype Controls |
Research Areas for KAP1 Antibody (NBP2-76398)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TRIM28 |
![Western Blot: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by WB of HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Lower panel shows WB for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-NBP2-76398-img0001.jpg "Western Blot: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by WB of HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Lower panel shows WB for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP1 (S824) in FFPE breast carcinoma by IHC and in FFPE EPE treated HeLa cells by ICC. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunocytochemistry-NBP2-76398-img0004.jpg "Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP1 (S824) in FFPE breast carcinoma by IHC and in FFPE EPE treated HeLa cells by ICC. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.")
![Immunohistochemistry: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunohistochemistry-NBP2-76398-img0002.jpg "Immunohistochemistry: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.")
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) (red) in formaldehyde-fixed asynchronous HeLa cells treated with EPE (right) or untreated (left) by ICC-IF. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin conjugated Alexafluor 488 (green).](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunocytochemistry-Immunofluorescence-NBP2-76398-img0003.jpg "Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) (red) in formaldehyde-fixed asynchronous HeLa cells treated with EPE (right) or untreated (left) by ICC-IF. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin conjugated Alexafluor 488 (green).")
![Immunoprecipitation: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phopsho KAP-1 (S824) by WB of immunoprecipitates from HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398) and rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411).](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunoprecipitation-NBP2-76398-img0005.jpg "Immunoprecipitation: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phopsho KAP-1 (S824) by WB of immunoprecipitates from HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398) and rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411).")
![Flow Cytometry- KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Antibody: Rabbitanti-phospho KAP-1 recombinant monoclonal[BL-246-7B5] or isotype control (unshaded).Secondary: DyLight® 488-conjugated goat anti-rabbit IgG](http://images.novusbio.com/fullsize/nbp2-76398_rabbit-monoclonal-kap1-p-ser824-antibody-bl-246-7b5-flow-cytometry-30112023101053..jpg "Flow Cytometry- KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Antibody: Rabbitanti-phospho KAP-1 recombinant monoclonal[BL-246-7B5] or isotype control (unshaded).Secondary: DyLight® 488-conjugated goat anti-rabbit IgG")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")