KAP1 Antibodies
We offer KAP1 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, RNA Inhibition, Simple Western, Western Blot. Each KAP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our KAP1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the KAP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our KAP1 polyclonal antibodies and browse our KAP1 monoclonal antibody catalog.
Alternate Names for KAP1 Antibodies
anti-KAP1 antibody, anti-TRIM28 antibody, anti-E3 SUMO-protein ligase TRIM28 antibody, anti-EC 6.3.2.- antibody, anti-FLJ29029 antibody, anti-KAP-1 antibody, anti-KAP1KRAB-associated protein 1 antibody, anti-KRIP-1 antibody, anti-Nuclear corepressor KAP-1 antibody, anti-RNF96KRAB-interacting protein 1 antibody, anti-TF1B antibody, anti-TIF1-beta antibody, anti-TIF1BRING finger protein 96 antibody, anti-transcription intermediary factor 1-beta antibody, anti-transcriptional intermediary factor 1-beta antibody, anti-tripartite motif containing 28 antibody, anti-tripartite motif-containing 28 antibody, anti-Tripartite motif-containing protein 28 antibody
17 Results for "kap1" in Primary Antibodies
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody (NB100-2350) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0019.jpg "Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody (NB100-2350) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2350-img0015.jpg)
![Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0014.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (15 ug) from NIH 3T3 cellstreated with 100 uM etoposide (+) or mock treated (-).Antibodies: Affinity purified rabbit anti-Phospho KAP-1(S824) antibody used forWB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 30 seconds. Lower panel shows westernblot for total KAP-1 using rabbit anti-KAP-1 recombinantmonoclonal antibody at 1:1000.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0017.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (50 ug) from HEK293Tcells treated with 100 uM etoposide (+) or mock treated(-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1(S824) antibody used forWB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second. Lower panel shows westernblot for total KAP-1 using affinity purified rabbit anti KAP-1 antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0018.jpg)
![Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) anduntreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0013.jpg)
![Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) and rabbit anti-KAP-1 recombinant monoclonal antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Phospho KAP-1 (S824), Antibody was used at 0.04 ug/ml. To examine total KAP-1, the blot was stripped and then blotted with this antibody at 1:1000 (lower panel). Detection: Chemiluminescence with an exposure time of 1 second.Previous](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunoprecipitation-NB100-2350-img0016.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MAGE I regulates KAP1 gene binding, trimethylation of histone 3 on lysine 9, & gene repression in HEK293T cells.MAGE I expression decreases binding of KAP1, H3me3K9, & repression of the ID1 tumor suppressor gene (A, B, D). In contrast, MAGE I expression increases binding of KAP1, H3me3K9, & repression of mRNA & protein levels of the Ki67 gene (E, F, H, I, J, L). Note MAGE I binds to Ki67 gene sites but not ID1 gene sites (C, G, K). “M” denotes Mock transfection control. “A3” & “C2” denote MAGE-A3 & MAGE-C2, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21876767), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2350_rabbit-polyclonal-kap1-p-ser824-antibody-310202415334921.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Truncated PPM1D impairs DNA damage response in mouse thymus. Expression of PPM1D mRNA analyzed by RT-qPCR in thymi of Ppm1d+/+, Ppm1dT/+ & Ppm1dT/T mice & normalized to GAPDH (n = 3) (A). Thymi from mice of indicated genotypes lysed & proteins separated by SDS-PAGE. Samples probed w/ antibody against PPM1D & importin-beta as a loading control. The empty & full arrowheads indicate the position of full-length & the C-terminally truncated PPM1D, respectively. (B). Cells from thymi from Ppm1d+/+ & Ppm1dT/+ mice analyzed by flow cytometry. Plotted are the counts of indicated populations as follows: double-negative T-cells (DN & DN1, DN2, DN3, DN4), double-positive T-cells (DP), CD8-positive T-cells (CD8+) & CD4-positive T-cells (CD4+) (n = 3) (C). The median size of thymus determined in Ppm1d +/+ (n = 11) & Ppm1dT/+ (n = 12) mice (D). A scheme of experimental setup in F-I. Mice exposed or not to a low dose of IR (3 Gy), sacrificed after 6 h & thymi & lymph nodes collected (E). Proteins isolated from thymi from mice of indicated genotypes exposed to mock or to IR probed w/ indicated antibodies by immunoblotting (F). Proteins isolated from inguinal lymph nodes from mice of indicated genotypes exposed to mock or to IR probed w/ indicated antibodies by immunoblotting (G). RNA isolated from thymi from mice in E analyzed by RT-qPCR. Expression of CDKN1Ap21 mRNA normalized to GAPDH. Statistical significance evaluated by two-tailed t-test, error bars indicate SD, n = 5 (H). RNA isolated from thymi from mice in D analyzed by RT-qPCR. Expression of PUMA mRNA normalized to GAPDH. Statistical significance evaluated by two-tailed t-test, error bars indicate SD, n = 5. * p <0.05; *** p < 0.0005; **** p < 0.0001 (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32927737), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2350_rabbit-polyclonal-kap1-p-ser824-antibody-3102024161262.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Truncated PPM1D impairs DNA damage response in mouse thymus. Expression of PPM1D mRNA analyzed by RT-qPCR in thymi of Ppm1d+/+, Ppm1dT/+ & Ppm1dT/T mice & normalized to GAPDH (n = 3) (A). Thymi from mice of indicated genotypes lysed & proteins separated by SDS-PAGE. Samples probed w/ antibody against PPM1D & importin-beta as a loading control. The empty & full arrowheads indicate the position of full-length & the C-terminally truncated PPM1D, respectively. (B). Cells from thymi from Ppm1d+/+ & Ppm1dT/+ mice analyzed by flow cytometry. Plotted are the counts of indicated populations as follows: double-negative T-cells (DN & DN1, DN2, DN3, DN4), double-positive T-cells (DP), CD8-positive T-cells (CD8+) & CD4-positive T-cells (CD4+) (n = 3) (C). The median size of thymus determined in Ppm1d +/+ (n = 11) & Ppm1dT/+ (n = 12) mice (D). A scheme of experimental setup in F-I. Mice exposed or not to a low dose of IR (3 Gy), sacrificed after 6 h & thymi & lymph nodes collected (E). Proteins isolated from thymi from mice of indicated genotypes exposed to mock or to IR probed w/ indicated antibodies by immunoblotting (F). Proteins isolated from inguinal lymph nodes from mice of indicated genotypes exposed to mock or to IR probed w/ indicated antibodies by immunoblotting (G). RNA isolated from thymi from mice in E analyzed by RT-qPCR. Expression of CDKN1Ap21 mRNA normalized to GAPDH. Statistical significance evaluated by two-tailed t-test, error bars indicate SD, n = 5 (H). RNA isolated from thymi from mice in D analyzed by RT-qPCR. Expression of PUMA mRNA normalized to GAPDH. Statistical significance evaluated by two-tailed t-test, error bars indicate SD, n = 5. * p <0.05; *** p < 0.0005; **** p < 0.0001 (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32927737), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2350_rabbit-polyclonal-kap1-p-ser824-antibody-3102024155451.jpg)
![Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NB500-158-img0028.jpg "Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158] - FFPE sections of human breast carcinoma. KAP1 antibody Lot3 (left) and Lot2 (right) used at a dilution of 1:1000 (1 ug/mL). Detection: DAB.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-NB500-158-img0022.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NB500-158] - Detection of Human KAP1 by Western blot. Whole cell lysate from HeLa (5, 15 and 50 ug), and 293T (50 ug) cells. KAP1 antibody at 0.1 ug/mL. Detection by chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NB500-158-img0024.jpg)
![Western Blot: KAP1 Antibody [NB500-158] - Hep3B human hepatocellular carcinoma cell line. 50 ug cell lysate per lane. 10% SDS-PAGE. KAP1 (TRIM28) antibody at 1:1000 dilution in 5% milk at 4C O/N. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NB500-158-img0025.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KAP1 Antibody [NB500-158] - Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NB500-158 (lot NB500-158-3) used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-158-2) and rabbit anti-KAP-1 antibody NB500-159. For blotting immunoprecipitated KAP-1, NB500-158 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NB500-158-img0026.jpg)
![Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76398_rabbit-monoclonal-kap1-p-ser824-antibody-bl-246-7b5-30320239244.jpg "Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![Western Blot: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by WB of HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Lower panel shows WB for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-NBP2-76398-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP1 (S824) in FFPE breast carcinoma by IHC and in FFPE EPE treated HeLa cells by ICC. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunocytochemistry-NBP2-76398-img0004.jpg)
![Immunohistochemistry: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunohistochemistry-NBP2-76398-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) (red) in formaldehyde-fixed asynchronous HeLa cells treated with EPE (right) or untreated (left) by ICC-IF. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin conjugated Alexafluor 488 (green).](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunocytochemistry-Immunofluorescence-NBP2-76398-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phopsho KAP-1 (S824) by WB of immunoprecipitates from HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398) and rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411).](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunoprecipitation-NBP2-76398-img0005.jpg)
![Flow Cytometry- KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Antibody: Rabbitanti-phospho KAP-1 recombinant monoclonal[BL-246-7B5] or isotype control (unshaded).Secondary: DyLight® 488-conjugated goat anti-rabbit IgG](http://images.novusbio.com/fullsize/nbp2-76398_rabbit-monoclonal-kap1-p-ser824-antibody-bl-246-7b5-flow-cytometry-30112023101053..jpg)
![Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in NIH/3T3.](http://images.novusbio.com/fullsize/KAP1-Antibody-4E6-Western-Blot-H00010155-M01-img0015.jpg "Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/KAP1-Antibody-4E6-Immunocytochemistry-Immunofluorescence-H00010155-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/KAP1-Antibody-4E6-Immunohistochemistry-Paraffin-H00010155-M01-img0011.jpg)
![Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi ( Cat # H00010155-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody (M01) clone 4E6 (Cat # H00010155-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/KAP1-Antibody-4E6-Western-Blot-H00010155-M01-img0012.jpg)
![Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in PC-12.](http://images.novusbio.com/fullsize/KAP1-Antibody-4E6-Western-Blot-H00010155-M01-img0013.jpg)
![Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6 Analysis of TRIM28 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/KAP1-Antibody-4E6-Western-Blot-H00010155-M01-img0014.jpg)
![Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M01), clone 4E6.Lane 1: TRIM28 transfected lysate(88.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/KAP1-Antibody-4E6-Western-Blot-H00010155-M01-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1161-img0004.jpg "Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: KAP1 Antibody [NB100-1161] - (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-Paraffin-NB100-1161-img0001.jpg)
![Flow Cytometry: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/KAP1-Antibody-Flow-Cytometry-NB100-1161-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NB100-1161] - analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1161-img0003.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249413344.jpg "Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249371524.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249393512.jpg)
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249374953.jpg)
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Human tissue extract (30 ug) was separatedby 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249374946.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-212024939154.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-212024940860.jpg)
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249383124.jpg)
![Western Blot: KAP1 Antibody [NB500-159] - Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody NB500-159 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NB500-159-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NB500-159] - Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody NB500-159 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of mouse plasmacytoma Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-NB500-159-img0006.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-159] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. Lot4 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-NB500-159-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KAP1 Antibody [NB500-159] - Detection of human KAP-1 by western blot of immunoprecipitates. Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NB500-159 (lot NB500-159-4) used for IP at 6 ug per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-159-3) and rabbit anti-KAP-1 antibody NB500-158. For blotting immunoprecipitated KAP-1, NB500-159 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NB500-159-img0011.jpg)
![Western Blot: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human and mouse KAP-1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/KAP1-Antibody-BL-248-2G6-Western-Blot-NBP2-76411-img0001.jpg "Western Blot: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human and mouse KAP-1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 lysate. Antibody: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human KAP-1 in FFPE HeLa cells by ICC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-Antibody-BL-248-2G6-Immunocytochemistry-NBP2-76411-img0004.jpg)
![Immunohistochemistry: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human KAP-1 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-Antibody-BL-248-2G6-Immunohistochemistry-NBP2-76411-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KAP1 Antibody (BL-248-2G6) [NBP2-76411] - Detection of human KAP-1 by WB of immunoprecipitates from HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411) and rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398).](http://images.novusbio.com/fullsize/KAP1-Antibody-BL-248-2G6-Immunoprecipitation-NBP2-76411-img0005.jpg)
![Western Blot: KAP1 Antibody (20C1) [NB100-74549] - Analysis of TIF1 beta in gamma irradiated HeLA cells.](http://images.novusbio.com/fullsize/KAP1-Antibody-20C1-Western-Blot-NB100-74549-img0005.jpg "Western Blot: KAP1 Antibody (20C1) [NB100-74549] - Analysis of TIF1 beta in gamma irradiated HeLA cells.")
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody (20C1) [NB100-74549] - Gamma irradiated HeLa cells.](http://images.novusbio.com/fullsize/KAP1-Antibody-20C1-Immunocytochemistry-Immunofluorescence-NB100-74549-img0004.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014] - Staining in human ovary and liver tissues using anti-TRIM28 antibody. Corresponding TRIM28 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-39014-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014] - Staining in human ovary and liver tissues using anti-TRIM28 antibody. Corresponding TRIM28 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NBP2-39014] - Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39014-img0004.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-39014-img0005.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-39014] - Staining of human ovary shows high expression.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-39014-img0006.jpg)
![Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NBP2-32072-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: KAP1 Antibody [NBP2-32072] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32072 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32072. For blotting immunoprecipitated KAP-1, NBP2-32072 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NBP2-32072-img0003.jpg)