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Integrin alpha 1/CD49a Antibody (CL7217)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7217) [NBP2-76479] - Analysis in human prostate and skeletal muscle tissues. Corresponding ITGA1 RNA-seq data are presented ...read more
Genetic Strategies: Western Blot: Integrin alpha 1/CD49a Antibody (CL7217) [NBP2-76479] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1, using Anti-ITGA1 antibody. ...read more
Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7217) [NBP2-76479] - Staining of human placenta shows strong membranous positivity in blood vessels, as well as positivity in loose connective tissue.
Western Blot: Integrin alpha 1/CD49a Antibody (CL7217) [NBP2-76479] - Analysis in human cell line SH-SY5Y.
Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7217) [NBP2-76479] - Staining of human prostate shows strong membranous positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7217) [NBP2-76479] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: Integrin alpha 1/CD49a Antibody (CL7217) [NBP2-76479] - Staining of human stomach shows moderate membranous positivity in glandular cells, as well as staining in basement membrane.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, KD
Clone
CL7217
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Integrin alpha 1/CD49a Antibody (CL7217) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RQISRSFFSGTQERKVQRN
Predicted Species
Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Mouse (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Integrin alpha 1/CD49a Antibody (CL7217)

  • CD49 antigen-like family member A
  • CD49a antigen
  • CD49a
  • Integrin alpha 1
  • integrin, alpha 1
  • ITGA1
  • Laminin and collagen receptor
  • very late activation protein 1
  • VLA-1
  • VLA1integrin alpha-1

Background

Integrin alpha 1 encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, KD

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ITGA1