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IL-10 Antibody [Unconjugated]

 (26 Publications)
 (1 Review)
 
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Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-10 Antigen ...read more
IL-10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) at 15 µg/mL overnight at 4 °C. Tissue ...read more
IL-10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) at 15 µg/mL overnight at 4 °C. Tissue ...read more
Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL‑10 ...read more
Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF217
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF-217-SP
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IL-10 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Specificity
Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant Epstein-Barr virus IL-10 is observed and less than 20% cross-reactivity with recombinant mouse IL‑10, recombinant feline IL-10, recombinant porcine IL‑10, recombinant equine IL-10,  and recombinant rat IL‑10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL10
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2-1.0 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
AF-217-NA in the following applications:
Publications
Read Publications using
AF-217-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody [Unconjugated]

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, IL-24, and IL-26/AK155 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature human IL-10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha , IL-22 R alpha , or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (11‑13). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  9. Syto, R. et al. (1998) Biochemistry 37:16943.
  10. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  11. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  12. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  13. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  14. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  15. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  16. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10 Antibody (AF-217-NA)(26)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 5 applications: Functional Assay, IHC, IHC-P, Neutralization, Western Blot.


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Functional Assay
(1)
IHC
(2)
IHC-P
(7)
Neutralization
(8)
Western Blot
(1)
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Filter By Species
Human
(17)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using AF-217-NA Applications Species
Cheng, YC;Acedera, JD;Li, YJ;Shieh, SY; A keratinocyte-adipocyte signaling loop is reprogrammed by loss of BTG3 to augment skin carcinogenesis Cell death and differentiation 2024-05-07 [PMID: 38714880] (Neutralization, Human) Neutralization Human
Papareddy, P;Tapken, I;Kroh, K;Varma Bhongir, RK;Rahman, M;Baumgarten, M;Cim, EI;Györffy, L;Smeds, E;Neumann, A;Veerla, S;Olinder, J;Thorlacus, H;Ryden, C;Bartakova, E;Holub, M;Herwald, H; The role of extracellular vesicle fusion with target cells in triggering systemic inflammation Nature communications 2024-02-07 [PMID: 38326335] (Functional Assay, Human) Functional Assay Human
Mick J.M. van Eijs, José J.M. Ter Linde, Matthijs J.D. Baars, Mojtaba Amini, Miangela M. Laclé, Eelco C. Brand et al. Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis iScience 2023-10-20 [PMID: 37766980]
A Dumigan, O Cappa, B Morris, J Sá Pessoa, R Calderon-G, G Mills, R Lancaster, D Simpson, A Kissenpfen, JA Bengoechea In vivo single-cell transcriptomics reveal Klebsiella pneumoniae skews lung macrophages to promote infection Embo Molecular Medicine, 2022-11-07;0(0):e16888. 11/7/2022 [PMID: 36337046]
P Shrestha, DA Davis, HK Jaeger, A Stream, AI Aisabor, R Yarchoan Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2 PloS Pathogens, 2021-01-07;17(1):e1009091. 1/7/2021 [PMID: 33411730]
NC Fay, BP Muthusamy, LP Nyugen, RC Desai, A Taverner, J MacKay, M Seung, T Hunter, K Liu, A Chandalia, MP Coyle, HL Kim, S Postlethwa, K Mangat, L Song, E Seto, A Alam, CV Olson, W Feng, M Saberi, TA Mahmood, RJ Mrsny A Novel Fusion of IL-10 Engineered to Traffic across Intestinal Epithelium to Treat Colitis J Immunol, 2020-11-04;0(0):. 2020-11-04 [PMID: 33148717] (Western Blot, Human) Western Blot Human
Zheng X, Fernando V, Sharma V et al. Correction of Arginine Metabolism with Sepiapterin-the Precursor of Nitric Oxide Synthase Cofactor BH4-Induces Immunostimulatory-shift of Breast Cancer Biochem. Pharmacol. 2/26/2020 [PMID: 32112882]
Expression of Tim-1 in primary CNS lymphoma Cancer Med, 2016-10-05;5(11):3235-3245. 2016-10-05 [PMID: 27709813] (IHC-P, Human) IHC-P Human
Role of Interleukin-10 on Nasal Polypogenesis in Patients with Chronic Rhinosinusitis with Nasal Polyps PLoS ONE, 2016-09-01;11(9):e0161013. 2016-09-01 [PMID: 27584662] (IHC-P, Human) IHC-P Human
Tobias Waller, Laura Kesper, Josefine Hirschfeld, Henrik Dommisch, Johanna Kölpin, Johannes Oldenburg et al. Porphyromonas gingivalis Outer Membrane Vesicles Induce Selective Tumor Necrosis Factor Tolerance in a Toll-Like Receptor 4- and mTOR-Dependent Manner Infection and Immunity 2016-04-01 [PMID: 26857578]
Show All 26 Publications.

Review for IL-10 Antibody (AF-217-NA) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF-217-NA:
Filter by Applications
IHC-Fr
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen IL-10 AF-217-NA
Enlarge 		3
reviewed by:
Tyler Crowe
 IHC-Fr Human 12/14/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
LotQW011812A

Comments

CommentsBlock with 5% BSA, incubated with antibody 1:50 in blocking solution at 37C for 1 hour

Product General Protocols

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FAQs for IL-10 Antibody (AF-217-NA) (0)

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Immunohistochemistry-Frozen IL-10 AF-217-NA
Enlarge
 Tyler Crowe
12/14/2018
Application: IHC-Fr
Species: Human
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Bioinformatics

Gene Symbol IL10
Uniprot