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Rat IL-1 RI ELISA Kit (Colorimetric)

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Sandwich ELISA: Rat IL-1 RI ELISA Kit (Colorimetric) [NBP2-80382] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Sandwich ELISA: Rat IL-1 RI ELISA Kit (Colorimetric) [NBP2-80382] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Conjugate
HRP
Suitable Sample Type
Serum
Standard Curve Range
54.69-3500 pg/ml (example only; lot dependent)
Sensitivity
14.76 pg/ml

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Rat IL-1 RI ELISA Kit (Colorimetric) Summary

Specificity
Recognizes both recombinant and natural Rat IL-1 RI.
Standard Curve Range
54.69-3500 pg/ml (example only; lot dependent)
Sensitivity
14.76 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 8.3 %
Intra-Assay
CV% < 3.1
Spike Recovery
124-129%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL1R1

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. 1. 96 well microplate coated with Capture Antibody
  2. 2. Detection Antibody conjugated to HRP
  3. 3. Standards
  4. 4. Wash Buffer Concentrate
  5. 5. Dilution Buffer Concentrate
  6. 6. Color Reagent A
  7. 7. Color Reagent B
  8. 8. Stop Solution

Alternate Names for Rat IL-1 RI ELISA Kit (Colorimetric)

  • CD121 antigen-like family member A
  • CD121a antigen
  • CD121a
  • IL-1 RI
  • IL1R
  • IL1R1
  • IL1RI
  • IL-1RI
  • IL1RT1
  • IL-1RT1
  • IL-1RT-1
  • Interleukin 1 Receptor 1
  • interleukin 1 receptor, type I
  • interleukin receptor 1
  • interleukin-1 receptor type 1
  • p80

Background

The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family (1). IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway (2-3). Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  Manual - Rat IL-1 RI ELISA Kit (Colorimetric)
  PDF Datasheet
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Bioinformatics

Gene Symbol IL1R1