IL-1 beta/IL-1F2 Antibody (8516) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Development, Flow Cytometry, ICC, IHC-P, Neutralization, Western Blot.

Showing Publications 1 - 10 of 63. Show All 63 Publications. Collapse Publications.

Publications using MAB201	Applications	Species
Bass, AR;Egan, MS;Alexander-Floyd, J;Lopes Fischer, N;Doerner, J;Shin, S; Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activation mBio 2023-09-22 [PMID: 37737612] (ICC, Human)	ICC	Human
Zhang, J;Brodsky, IE;Shin, S; Yersinia deploys type III-secreted effectors to evade caspase-4 inflammasome activation in human cells mBio 2023-08-24 [PMID: 37615436] (Western Blot, Human)	Western Blot	Human
Smer-Barreto, V;Quintanilla, A;Elliott, RJR;Dawson, JC;Sun, J;Campa, VM;Lorente-Macías, Á;Unciti-Broceta, A;Carragher, NO;Acosta, JC;Oyarzún, DA; Discovery of senolytics using machine learning Nature communications 2023-06-10 [PMID: 37301862] (ICC, Western Blot, Human)	ICC, Western Blot	Human
Yu-Chuan Lin, Chun-Hung Hua, Hsin-Man Lu, Shi-Wei Huang, Yeh Chen, Ming-Hsui Tsai et al. CAR-T cells targeting HLA-G as potent therapeutic strategy for EGFR-mutated and overexpressed oral cancer iScience 3/17/2023 [PMID: 36876120]
J Zhang, IE Brodsky, S Shin Yersinia Type III-Secreted Effectors Subvert Caspase-4-dependent Inflammasome Activation in Human Cells bioRxiv : the preprint server for biology, 2023-01-25;0(0):. 2023-01-25 [PMID: 36747770] (Western Blot, Human)	Western Blot	Human
Zhouyangfan Peng, Xiqing Tan, Liangpeng Xie, Ze Li, Sufang Zhou, Yapei Li PKR deficiency delays vascular aging via inhibiting GSDMD-mediated endothelial cell hyperactivation iScience 1/20/2023 [PMID: 36691613]
Haoran Zhu, Keefe T Chan, Xinran Huang, Carmelo Cerra, Shaun Blake, Anna S Trigos et al. Cystathionine-beta -synthase is essential for AKT-induced senescence and suppresses the development of gastric cancers with PI3K/AKT activation eLife 6/27/2022 [PMID: 35758651]
Rajia Bahri, Orsolya Kiss, Ian Prise, Karen M. Garcia-Rodriguez, Haris Atmoko, Julia M. Martínez-Gómez et al. Human Melanoma-Associated Mast Cells Display a Distinct Transcriptional Signature Characterized by an Upregulation of the Complement Component 3 That Correlates With Poor Prognosis Frontiers in Immunology 5/20/2022 [PMID: 35669782]
Xiuxing Liu, Jianjie Lv, Huishi Wang, Yingfeng Zheng, Wenru Su Functional analysis of human circulating immune cells based on high-dimensional mass cytometry STAR Protocols 6/1/2022 [PMID: 35434658]
Y Li, Y Zhu, S Feng, Y Ishida, TP Chiu, T Saito, S Wang, DK Ann, JJ Ou Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1beta to downregulate PPARalpha and FOXO3 Cell Reports, 2022-01-25;38(4):110284. 2022-01-25 [PMID: 35081341] (Neutralization, Human)	Neutralization	Human
Yapei Li, Ying Li, Lijun Li, Minghui Yin, Jiangang Wang, Xiaohui Li PKR deficiency alleviates pulmonary hypertension via inducing inflammasome adaptor ASC inactivation Pulmonary Circulation 10/1/2021 [PMID: 34540200]
Vijayashree Mysore, Xavier Cullere, Matthew L. Settles, Xinge Ji, Michael W. Kattan, Michaël Desjardins et al. Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigens Med (N Y) 9/1/2021 [PMID: 34414383]
K Tsuchiya, S Hosojima, H Hara, H Kushiyama, MR Mahib, T Kinoshita, T Suda Gasdermin D mediates the maturation and release of IL-1&alpha downstream of inflammasomes Cell Reports, 2021-03-23;34(12):108887. 2021-03-23 [PMID: 33761363] (Western Blot, Mouse)	Western Blot	Mouse
VM Abrahams, Z Tang, G Mor, S Guller NLRP3 inflammasome function and pyroptotic cell death in human placental Hofbauer cells J Reprod Immunol, 2020-10-03;142(0):103214. 2020-10-03 [PMID: 33152658] (Western Blot, Human)	Western Blot	Human
S Lichtenegg, J Stiehler, S Saiger, A Zauner, B Kleinhappl, C Bernecker, P Schlenke, GE Wagner, K Krause, M Gastager, I Steinmetz Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model PLoS Negl Trop Dis, 2020-11-02;14(11):e0008840. 2020-11-02 [PMID: 33137811] (Western Blot, Human)	Western Blot	Human
H Israelov, O Ravid, D Atrakchi, D Rand, S Elhaik, Y Bresler, R Twitto-Gre, L Omesi, S Liraz-Zalt, F Gosselet, M Schnaider, I Cooper Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair J Neuroinflammation, 2020-09-09;17(1):267. 2020-09-09 [PMID: 32907600] (Neutralization, Human)	Neutralization	Human
Paul Hendrix, Zhonghua Tang, Michelle Silasi, Karen E. Racicot, Gil Mor, Vikki M. Abrahams et al. Herpesvirus-infected Hofbauer cells activate endothelial cells through an IL-1 beta -dependent mechanism Placenta 2/1/2020 [PMID: 32174308]
S Pathak, A Vambutas Autoimmune inner ear disease patient-associated 28-kDa proinflammatory IL-1beta fragment results from caspase-7-mediated cleavage in vitro JCI Insight, 2020-02-13;5(3):. 2020-02-13 [PMID: 32051334] (Western Blot, Human)	Western Blot	Human
Takashi Maehara, Naoki Kaneko, Cory A. Perugino, Hamid Mattoo, Jesper Kers,, Hugues Allard-Chamard et al. Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis Journal of Clinical Investigation 4/6/2020 [PMID: 31990684]
R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling Nat Commun, 2019-11-01;10(1):5016. 2019-11-01 [PMID: 31676788] (Neutralization, Human)	Neutralization	Human
Fan Yang, Anqi Li, Ying Qin, Hui Che, Yueqiu Wang, Jie Lv et al. A Novel Circular RNA Mediates Pyroptosis of Diabetic Cardiomyopathy by Functioning as a Competing Endogenous RNA Molecular Therapy - Nucleic Acids 9/6/2019 [PMID: 31400606]
Hongxiao Song, Guangyun Tan, Yang Yang, An Cui, Haijun Li, Tianyang Li et al. Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes The Journal of Immunology 4/15/2019 [PMID: 30842274]
Xiulei Mo, Cong Tang, Qiankun Niu, Tingxuan Ma, Yuhong Du, Haian Fu HTiP: High-Throughput Immunomodulator Phenotypic Screening Platform to Reveal IAP Antagonists as Anti-cancer Immune Enhancers Cell Chemical Biology 3/1/2019 [PMID: 30639259]
Livingstone Fultang, Laura D. Gamble, Luciana Gneo, Andrea M. Berry, Sharon A. Egan, Fenna De Bie et al. Macrophage-derived IL-1 beta and TNF-alpha regulate arginine metabolism in neuroblastoma Cancer Research 2/1/2019 [PMID: 30545920]
K Vogel, M Pierau, A Arra, K Lampe, D Schlueter, C Arens, MC Brunner-We Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus Sci Rep, 2018-11-15;8(1):16904. 2018-11-15 [PMID: 30442915] (Neutralization, Human)	Neutralization	Human
DM Clancy, GP Sullivan, HBT Moran, CM Henry, EP Reeves, NG McElvaney, EC Lavelle, SJ Martin Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing Cell Rep, 2018-03-13;22(11):2937-2950. 2018-03-13 [PMID: 29539422] (Western Blot, Human)	Western Blot	Human
SEJ Chambers, CL O'Neill, J Guduric-Fu, KJ McLoughlin, A Liew, AM Egan, T O'Brien, AW Stitt, RJ Medina The Vasoreparative Function of Myeloid Angiogenic Cells Is Impaired in Diabetes Through the Induction of IL1? Stem Cells, 2018-03-09;36(6):834-843. 2018-03-09 [PMID: 29484768] (Neutralization, Western Blot, Human)	Neutralization, Western Blot	Human
Y Uchino, AM Woodward, J Mauris, K Peterson, P Verma, UJ Nilsson, J Rajaiya, P Argüeso Galectin-3 is an amplifier of the interleukin-1?-mediated inflammatory response in corneal keratinocytes Immunology, 2018-02-15;0(0):. 2018-02-15 [PMID: 29359328] (Western Blot, Human)	Western Blot	Human
Hye-Eun Byeon, Ja Young Jeon, Hae Jin Kim, Dae Jung Kim, Kwan-Woo Lee, Yup Kang et al. MicroRNA-132 Negatively Regulates Palmitate-Induced NLRP3 Inflammasome Activation through FOXO3 Down-Regulation in THP-1 Cells Nutrients 12/18/2017 [PMID: 29258239]
Alma-Martina Cepika, Romain Banchereau, Elodie Segura, Marina Ohouo, Brandi Cantarel, Kristina Goller et al. A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis Journal of Experimental Medicine 11/6/2017 [PMID: 28935693]
F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression Cancer Res., 2017-07-28;0(0):. 2017-07-28 [PMID: 28754674] (Neutralization, Human)	Neutralization	Human
N Nishijima, T Hirai, K Misato, M Aoyama, E Kuroda, KJ Ishii, K Higashisak, Y Yoshioka, Y Tsutsumi Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific Front Immunol, 2017-04-03;8(0):379. 2017-04-03 [PMID: 28421077] (Western Blot, Mouse)	Western Blot	Mouse
E Naschberge, W Gei beta dörfer, C Bogdan, P Tripal, E Kremmer, M Stürzl, N Britzen-La Processing and secretion of guanylate binding protein-1 depend on inflammatory caspase activity J. Cell. Mol. Med, 2017-03-08;0(0):. 2017-03-08 [PMID: 28272793] (Western Blot, Human)	Western Blot	Human
M Garstkiewi, GE Strittmatt, S Grossi, J Sand, G Fenini, S Werner, LE French, HD Beer Opposing effects of Nrf2 and Nrf2-activating compounds on the NLRP3 inflammasome independent of Nrf2-mediated gene expression Eur. J. Immunol, 2017-04-10;0(0):. 2017-04-10 [PMID: 28247911]
Li Y, Zheng JY, Liu JQ et al. Succinate/NLRP3 Inflammasome Induces Synovial Fibroblast Activation: Therapeutical Effects of Clematichinenoside AR on Arthritis. Front Immunol. 12/22/2016 [PMID: 28003810]
Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability Sci Rep, 2016-12-15;6(0):39035. 2016-12-15 [PMID: 27976724] (Western Blot, Human)	Western Blot	Human
R A Steinman Downregulation of estrogen receptor and modulation of growth of breast cancer cell lines mediated by paracrine stromal cell signals Breast Cancer Res. Treat., 2016-11-16;0(0):. 2016-11-16 [PMID: 27853906] (ICC, Human)	ICC	Human
NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis Arch Med Sci, 2016-07-20;12(5):950-958. 2016-07-20 [PMID: 27695484] (Western Blot, Human)	Western Blot	Human
Jong-Seok Moon, Kiichi Nakahira, Kuei-Pin Chung, Gina M. DeNicola, Michael Jakun Koo, Maria A. Pabón et al. NOX4-dependent fatty acid oxidation promotes NLRP3 inflammasome activation in macrophages Nature Medicine 9/1/2016 [PMID: 27455510]
Wan-Chien Cheng Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses Eur J Immunol, 2016-08-01;0(0):. 2016-08-01 [PMID: 27334899] (Neutralization, Human)	Neutralization	Human
Oliver R, Lovric V, Yu Y, Christou C, Aiken S, Cooper J, Walsh W Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue. PLoS ONE, 2015-08-25;10(8):e0136514. 2015-08-25 [PMID: 26305692] (IHC-P, Mouse)	IHC-P	Mouse
Bernard Dib, Haijiang Lin, Daniel E. Maidana, Bo Tian, John B. Miller, Peggy Bouzika et al. Mitochondrial DNA has a pro-inflammatory role in AMD Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 11/1/2015 [PMID: 26305120]
Alfaidi M, Wilson H, Daigneault M, Burnett A, Ridger V, Chamberlain J, Francis S Neutrophil elastase promotes interleukin-1beta secretion from human coronary endothelium. J Biol Chem, 2015-08-12;290(40):24067-78. 2015-08-12 [PMID: 26269588] (Neutralization, Human)	Neutralization	Human
Ansari M, Dutta S, Veettil M, Dutta D, Iqbal J, Kumar B, Roy A, Chikoti L, Singh V, Chandran B Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-beta Responses. PLoS Pathog, 2015-07-02;11(7):e1005019. 2015-07-02 [PMID: 26134128] (Western Blot, Human)	Western Blot	Human
Lili Wang, Peng Qu, Jian Zhao, Yeting Chang NLRP3 and downstream cytokine expression elevated in the monocytes of patients with coronary artery disease Archives of Medical Science 8/29/2014 [PMID: 25276166]
Yijun Hu, Haijiang Lin, Bernard Dib, Alp Atik, Peggy Bouzika, Christopher Lin et al. Cholesterol crystals induce inflammatory cytokines expression in a human retinal pigment epithelium cell line by activating the NF-kappa B pathway Discov Med 8/26/2015 [PMID: 25091484]
Nieminen J, Sipponen T, Farkkila M, Vaarala O Monocyte-derived dendritic cells from Crohn's disease patients exhibit decreased ability to activate T helper type 17 responses in memory cells. Clin Exp Immunol, 2014-07-01;177(1):190-202. 2014-07-01 [PMID: 24635023] (Flow Cytometry, Human)	Flow Cytometry	Human
Anne Walter, Matthias Schäfer, Virginia Cecconi, Claudia Matter, Mirjana Urosevic-Maiwald, Benedetta Belloni et al. Aldara activates TLR7-independent immune defence Nature Communications 3/5/2013 [PMID: 23463003]
Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 2012-09-25;10(9):e1001395. 2012-09-25 [PMID: 23055831] (Neutralization, Human)	Neutralization	Human
Hubo M et al. Plasmacytoid dendritic cells are inefficient in activation of human regulatory T cells. PLoS One 1/1/2012 [PMID: 22952871]
Nicolaidou V, Wong MM, Redpath AN Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. PLoS ONE, 2012-07-03;7(7):e39871. 2012-07-03 [PMID: 22802946] (Neutralization, Human)	Neutralization	Human
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012-04-26;484(7395):514-8. 2012-04-26 [PMID: 22466287] (Neutralization, Human)	Neutralization	Human
Gabriel Sollberger, Gerhard E. Strittmatter, Magdalena Kistowska, Lars E. French, Hans-Dietmar Beer Caspase-4 Is Required for Activation of Inflammasomes The Journal of Immunology 2/15/2012 [PMID: 22246630]
Abdallah M. Abdallah, Jovanka Bestebroer, Nigel D. L. Savage, Karin de Punder, Maaike van Zon, Louis Wilson et al. Mycobacterial Secretion Systems ESX-1 and ESX-5 Play Distinct Roles in Host Cell Death and Inflammasome Activation The Journal of Immunology 11/1/2011 [PMID: 21957139]
John G. Walsh, Susan E. Logue, Alexander U. Lüthi, Seamus J. Martin Caspase-1 Promiscuity Is Counterbalanced by Rapid Inactivation of Processed Enzyme* Journal of Biological Chemistry 9/16/2011 [PMID: 21757759]
Jon R. Ward, Peter W. West, Mark P. Ariaans, Lisa C. Parker, Sheila E. Francis, David C. Crossman et al. Temporal interleukin-1beta secretion from primary human peripheral blood monocytes by P2X7-independent and P2X7-dependent mechanisms Journal of Biological Chemistry 7/23/2010 [PMID: 20495003]
Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Eur. J. Immunol., 2009-05-01;39(5):1221-30. 2009-05-01 [PMID: 19350551] (Neutralization, Human)	Neutralization	Human
Li J, Yin HL, Yuan J Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity. J. Cell Biol., 2008-04-14;181(2):321-33. 2008-04-14 [PMID: 18411310] (Western Blot, Human)	Western Blot	Human
Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol., 2007-08-05;8(9):942-9. 2007-08-05 [PMID: 17676045] (Neutralization, Human)	Neutralization	Human
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007-03-30;52(10):2775-83. 2007-03-30 [PMID: 17394066] (Neutralization, Human)	Neutralization	Human
Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway. Am. J. Respir. Cell Mol. Biol., 2006-05-11;35(4):407-14. 2006-05-11 [PMID: 16690986] (Neutralization, Human)	Neutralization	Human
Banks WA, Farr SA, La Scola ME Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum. J. Pharmacol. Exp. Ther., 2001-11-01;299(2):536-41. 2001-11-01 [PMID: 11602664] (Neutralization, Human)	Neutralization	Human
Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia. J. Neurochem., 2000-03-01;74(3):1017-25. 2000-03-01 [PMID: 10693932] (ELISA Development, Human)	ELISA Development	Human
Show All 63 Publications. Collapse Publications.

Array MAB201

• IL-1 beta/IL-1F2 Antibodies
• IL-1 beta/IL-1F2 ELISA Kit
• IL-1 beta/IL-1F2 Lysate
• IL-1 beta/IL-1F2 Proteins
• IL-1 beta/IL-1F2 ELISAs
• IL-1 beta/IL-1F2 Proteins and Enzymes