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IL-1 beta/IL-1F2 Antibody (8516) [Allophycocyanin]

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Peripheral blood mononuclear cell monocytes, resting (open histogram), or LPS-treated (filled histogram) were stained with Mouse Anti-Human IL-1 beta /IL-1F2 APC-conjugated Monoclonal Antibody (Catalog # IC201A) or isotype control antibody (Catalog # IC002A). View our protocol for Staining Intracellular Molecules.
Peripheral blood mononuclear cell monocytes, resting (open histogram), or LPS-treated (filled histogram) were stained with Mouse Anti-Human IL-1 beta /IL-1F2 APC-conjugated Monoclonal Antibody (Catalog # IC201A) or ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
8516
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC201U-100UG
Alexa Fluor 350
FAB201U
Alexa Fluor 405
FAB201V
Alexa Fluor 405
IC201V-100UG
Alexa Fluor 488
IC201G-100UG
Alexa Fluor 488
FAB201G
Alexa Fluor 532
FAB201X
Alexa Fluor 594
FAB201T
Alexa Fluor 594
IC201T-100UG
Alexa Fluor 647
IC201R-100UG
Alexa Fluor 647
FAB201R
Alexa Fluor 700
FAB201N
Alexa Fluor 700
IC201N-100UG
Alexa Fluor 750
FAB201S
Alexa Fluor 750
IC201S-100UG
Allophycocyanin
FAB201A
Biotin
FAB201B
DyLight 350
FAB201D
DyLight 405
FAB201E
DyLight 488
FAB201K
DyLight 550
FAB201L
DyLight 594
FAB201M
DyLight 650
FAB201W
DyLight 680
FAB201Y
DyLight 755
FAB201Z
Fluorescein
IC201F
HRP
FAB201H
Janelia Fluor 549
FAB201I
Janelia Fluor 646
FAB201J
PerCP (Peridinin-chlorophyll Protein Complex)
IC201C
Phycoerythrin
IC201P
Unconjugated
MAB201-100
mFluor Violet 450 SE
FAB201MFV450
mFluor Violet 500 SE
FAB201MFV500
mFluor Violet 610 SE
FAB201MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB201-100
Carrier Free
IC201C
Carrier Free
IC201F
Carrier Free
IC201P
Carrier Free
MAB201-SP
Carrier Free
MAB201-100
Carrier Free
MAB201-500
Carrier Free
IC201V-100UG
Carrier Free
IC201U-100UG
Carrier Free
IC201T-100UG
Carrier Free
IC201S-100UG
Carrier Free
IC201R-100UG
Carrier Free
IC201N-100UG
Carrier Free
IC201G-100UG
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IL-1 beta/IL-1F2 Antibody (8516) [Allophycocyanin] Summary

Immunogen
E. coli-derived recombinant human IL-1 beta /IL-1F2
aa 117-269
Accession # P01584
Specificity
Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and rpIL‑1 beta and no cross-reactivity with rrIL‑1 beta , rmIL‑1 alpha , rhIL‑1ra, rmIL‑1ra, or rrIL‑1 alpha .
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL1B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using
IC201A in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody (8516) [Allophycocyanin]

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 beta/IL-1F2 Antibody (IC201A)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol IL1B
Uniprot