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IGF-I/IGF-1 Antibody (SPM406) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM406
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34409AF350
Alexa Fluor 405
NBP2-34409AF405
Alexa Fluor 488
NBP2-34409AF488
Alexa Fluor 532
NBP2-34409AF532
Alexa Fluor 594
NBP2-34409AF594
Alexa Fluor 647
NBP2-34409AF647
Alexa Fluor 700
NBP2-34409AF700
Alexa Fluor 750
NBP2-34409AF750
Allophycocyanin
NBP2-34409APC
Biotin
NBP2-34409B
DyLight 350
NBP2-34409UV
DyLight 405
NBP2-34409V
DyLight 488
NBP2-34409G
DyLight 550
NBP2-34409R
DyLight 594
NBP2-34409DL594
DyLight 650
NBP2-34409C
DyLight 680
NBP2-34409FR
DyLight 755
NBP2-34409IR
FITC
NBP2-34409F
HRP
NBP2-34409H
Janelia Fluor 525
NBP2-34409JF525
Janelia Fluor 549
NBP2-34409JF549
Janelia Fluor 646
NBP2-34409JF646
Janelia Fluor 669
NBP2-34409JF669
PE
NBP2-34409PE
PerCP
NBP2-34409PCP
Unconjugated
NBP2-34361-0.1mg
mFluor Violet 450 SE
NBP2-34409MFV450
mFluor Violet 500 SE
NBP2-34409MFV500
mFluor Violet 610 SE
NBP2-34409MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-34361-0.1mg
Azide and BSA Free
NBP2-34409-0.1mg
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IGF-I/IGF-1 Antibody (SPM406) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Purified human IGF-I/IGF-1 protein (Uniprot: P05019)
Localization
Cytoplasmic (Secreted)
Specificity
This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGF1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for IGF-I/IGF-1 Antibody (SPM406) [CoraFluor™ 1]

  • IBP1
  • IGF1
  • IGF-1
  • IGF1A
  • IGFI
  • IGF-I
  • IGF-IA
  • IGF-IB
  • insulin-like growth factor 1 (somatomedin C)
  • insulin-like growth factor 1
  • insulin-like growth factor I
  • insulin-like growth factor IA
  • insulin-like growth factor IB
  • Mechano growth factor
  • MGF
  • Somatomedin A
  • Somatomedin C
  • somatomedin-C

Background

Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IGF-I/IGF-1
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NBP2-34409CL1
IGF-I/IGF-1 Antibody (SPM406) [CoraFluor™ 1]
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol IGF1