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Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)

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Sandwich ELISA: Monkey ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-92712] - Representative standard curve for monkey ICAM-1 ELISA. Monkey ICAM-1 was diluted in serial 2-fold steps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed.
Sandwich ELISA: Monkey ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-92712] - Representative standard curve for monkey ICAM-1 ELISA. Monkey ICAM-1 was diluted in serial 2-fold steps in Sample Diluent. Do not use this ...read more

Product Details

Summary
Reactivity PmSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum (baboon, rhesus serum)
Standard Curve Range
6.3 - 100 U/mL
Sensitivity
1.1 U/mL

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Monkey ICAM-1/CD54 ELISA Kit (Colorimetric) Summary

Description
The monkey sICAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sICAM-1
Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a monkey ICAM-1 positive serum. There was no cross reactivity detected.
Standard Curve Range
6.3 - 100 U/mL
Sensitivity
1.1 U/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 5.0%
Spike Recovery
The overall mean recovery was 95%. Please see protocol for more specific Spike Recovery details.
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ICAM1

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
Publications
Read Publication using NBP1-92712.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Plate Covers
  2. Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to monkey sICAM-1 and HRP-Conjugate (anti-monkey sICAM-1 monoclonal (murine) antibody, Sample Diluent lyophilized
  3. Aluminium pouch(es) with a monkey sICAM-1 Standard curve (coloured)
  4. Sample Diluent (Use when an external predilution of the samples is needed)
  5. Stop Solution (1M Phosphoric acid)
  6. Substrate Solution (tetramethyl-benzidine)
  7. Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)

Alternate Names for Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

ntercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-beta-complex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. This family consists of the leukocyte adhesion glycoproteins LFA-1 which mediates lymphocyte adhesion, Mac-1 which mediates granulocyte adhesion and p150,95. ICAM-1 is a single-chain glycoprotein with a polypeptide core that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR. The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for ICAM-1/CD54 ELISA Kit (NBP1-92712)(1)

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  Manual - Human, Monkey ICAM-1/CD54 ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol ICAM1