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ICAM-1/CD54 Antibody [Unconjugated]

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Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) followed by HRP-conjugated Anti-Goat ...read more
ICAM‑1/CD54 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) at 5 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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ICAM-1/CD54 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat ICAM-1/CD54
Gln28-Thr493
Accession # Q00238
Specificity
Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) ICAM-1 and 5% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM‑3, and rmICAM‑2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ICAM1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Adhesion Blockade
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
AF583 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody [Unconjugated]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta , and lipolysaccharide (LPS). Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-1/CD54 Antibody (AF583)(26)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 3 applications: Flow Cytometry, IHC, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC
(4)
Western Blot
(6)
All Applications
Filter By Species
Mouse
(3)
Rat
(8)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using AF583 Applications Species
M Jiang, H Xie, C Zhang, T Wang, H Tian, L Lu, JY Xu, GT Xu, L Liu, J Zhang Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy Journal of Cellular and Molecular Medicine, 2022-01-11;0(0):. 2022-01-11 [PMID: 35023309] (Western Blot, Rat) Western Blot Rat
Shi FJ, Xie H, Zhang CY et al. Is Iba-1 protein expression a sensitive marker for microglia activation in experimental diabetic retinopathy? International journal of ophthalmology 2/18/2021 [PMID: 33614447]
Xu J, Yu C, Luo J, et al. The role and mechanism of the annexin A1 peptide Ac2-26 in rats with cardiopulmonary bypass lung injury Basic & clinical pharmacology & toxicology 1/17/2021 [PMID: 33455036]
Jinjie Liu, Zanhua Liu, Guoliang Liu, Kai Gao, Hengjie Zhou, Yongbo Zhao et al. Spinal cord injury and its underlying mechanism in rats with temporal lobe epilepsy Experimental and Therapeutic Medicine 1/15/2020 [PMID: 32104272]
RC Krolewski, B Lin, S Stampfer, A Packard, JE Schwob A Group of Olfactory Receptor Alleles that Encode Full Length Proteins are Down-Regulated as Olfactory Sensory Neurons Mature Sci Rep, 2020-02-04;10(1):1781. 2020-02-04 [PMID: 32020011] (Flow Cytometry, Mouse) Flow Cytometry Mouse
CW Lin, CM Yang, CH Yang Effects of the Emitted Light Spectrum of Liquid Crystal Displays on Light-Induced Retinal Photoreceptor Cell Damage Int J Mol Sci, 2019-05-10;20(9):. 2019-05-10 [PMID: 31083373] (Western Blot, Mouse) Western Blot Mouse
Nan-Fu Chen, Chun-Sung Sung, Zhi-Hong Wen, Chun-Hong Chen, Chien-Wei Feng, Han-Chun Hung et al. Therapeutic Effect of Platelet-Rich Plasma in Rat Spinal Cord Injuries Frontiers in Neuroscience 4/23/2018 [PMID: 29740270]
SA Bloomer, KE Wellen, GC Henderson Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice Lipids Health Dis, 2017-12-13;16(1):243. 2017-12-13 [PMID: 29237473] (Western Blot, Mouse) Western Blot Mouse
Jesus H. Dominguez, Yunlong Liu, Hongyu Gao, James M. Dominguez, Danhui Xie, K. J. Kelly Renal Tubular Cell-Derived Extracellular Vesicles Accelerate the Recovery of Established Renal Ischemia Reperfusion Injury Journal of the American Society of Nephrology 12/1/2017 [PMID: 28747315]
J Huang, S Han, Q Sun, Y Zhao, J Liu, X Yuan, W Mao, B Peng, W Liu, J Yin, X He Kv1.3 channel blocker (ImKTx88) maintains blood-brain barrier in experimental autoimmune encephalomyelitis Cell Biosci, 2017-06-07;7(0):31. 2017-06-07 [PMID: 28596825] (IHC, Rat) IHC Rat
Show All 26 Publications.

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Bioinformatics

Gene Symbol ICAM1
Uniprot