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HIF-2 alpha/EPAS1 Antibody

 (11 Publications)
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Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 h. PVDF membrane was probed with 1 µg/mL of Human/Rat HIF-2a/EPAS1 Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF-2a/EPAS1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with 150 µM CoCl2for 16 h. PVDF membrane was probed with 1 µg/mL of ...read more
BG01V human embryonic stem cells treated with 150 μM CoCl2 overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF‑2 alpha /EPAS1/DNA complexes were ...read more
The DU145FP cells express higher levels of cancer stemness related genes. (A) Transcriptome analyses of prostate cancer stemness-related markers. The Log2 transformed ratio of FPKM values (e.g., FP/WT) are indicated by ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ChIP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF2886-SP
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HIF-2 alpha/EPAS1 Antibody Summary

Immunogen
E. coli-derived recombinant human HIF‑2 alpha /EPAS1
Ser543-Thr870
Accession # Q99814
Specificity
Detects human and rat HIF‑2 alpha /EPAS1.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPAS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2886 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIF-2 alpha/EPAS1 Antibody

  • Basic-helix-loop-helix-PAS protein MOP2
  • BHLHE73
  • Class E basic helix-loop-helix protein 73
  • ECYT4
  • endothelial PAS domain protein 1
  • endothelial PAS domain-containing protein 1
  • EPAS1
  • EPAS-1
  • HIF 2A
  • HIF-1-alpha-like factor
  • HIF-1alpha-like factor
  • HIF2 alpha
  • HIF-2 alpha
  • hif2a angiogenesis
  • HIF2A
  • HIF-2-alpha
  • HIF2-alpha
  • HLF
  • hypoxia-inducible factor 2 alpha
  • Hypoxia-inducible factor 2-alpha
  • Member of PAS protein 2
  • MOP2
  • PAS domain-containing protein 2
  • PASD2

Background

The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha , complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIF-2 alpha/EPAS1 Antibody (AF2886)(11)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: ChIP, Western Blot.


Filter By Application
ChIP
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(6)
Rat
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF2886 Applications Species
Ioana V. Militaru, Alina Adriana Rus, Cristian V.A. Munteanu, Georgiana Manica, Stefana M. Petrescu New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma Frontiers in Oncology 2023-01-26 [PMID: 36776379]
X Ma, Z Tan, Q Zhang, K Ma, J Xiao, X Wang, Y Wang, M Zhong, Y Wang, J Li, X Zeng, W Guan, S Wang, K Gong, GH Wei, Z Wang VHL Ser65 mutations enhance HIF2alpha signaling and promote epithelial-mesenchymal transition of renal cancer cells Cell & bioscience, 2022-05-03;12(1):52. 2022-05-03 [PMID: 35505422] (ChIP, Human) ChIP Human
O Uchenunu, AV Zhdanov, P Hutton, P Jovanovic, Y Wang, DE Andreev, L Hulea, DJ Papadopoli, D Avizonis, PV Baranov, MN Pollak, DB Papkovsky, I Topisirovi Mitochondrial complex IV defects induce metabolic and signaling perturbations that expose potential vulnerabilities in HCT116 cells FEBS Open Bio, 2022-04-01;0(0):. 2022-04-01 [PMID: 35302710] (Western Blot, Human) Western Blot Human
AS Clees, V Stolp, B Häupl, DC Fuhrmann, F Wempe, M Seibert, S Weber, A Banning, R Tikkanen, R Williams, B Brüne, H Serve, F Schnütgen, I von Metzle, N Kurrle Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target Gene Cells, 2022-01-15;11(2):. 2022-01-15 [PMID: 35053409] (Western Blot, Human) Western Blot Human
Ughetta del Balzo, Pierre E. Signore, Gail Walkinshaw, Todd W. Seeley, Mitchell C. Brenner, Qingjian Wang et al. Nonclinical Characterization of the Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitor Roxadustat, a Novel Treatment of Anemia of Chronic Kidney Disease Journal of Pharmacology and Experimental Therapeutics 2020-08-01 [PMID: 32487538]
Manik C. Ghosh, De-Liang Zhang, Hayden Ollivierre, Michael A. Eckhaus, Tracey A. Rouault Translational repression of HIF2 alpha expression in mice with Chuvash polycythemia reverses polycythemia Journal of Clinical Investigation 2018-04-02 [PMID: 29480820]
MAS Martín-Ara, MT Rae, MG Darlison, AV Poole, JA Fraser Preferential activation of HIF-2? adaptive signalling in neuronal-like cells in response to acute hypoxia PLoS ONE, 2017-10-02;12(10):e0185664. 2017-10-02 [PMID: 28968430] (Western Blot, Human) Western Blot Human
X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells Sci Rep, 2017-07-11;7(1):5081. 2017-07-11 [PMID: 28698547] (Western Blot, Human) Western Blot Human
Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41 Nat Commun, 2016-07-07;7(0):12098. 2016-07-07 [PMID: 27384883] (Western Blot, Human) Western Blot Human
Zhdanov AV, Dmitriev RI, Papkovsky DB. Bafilomycin A1 activates HIF-dependent signalling in human colon cancer cells via mitochondrial uncoupling. Biosci Rep 12/1/2012 [PMID: 22943412]
Show All 11 Publications.

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Secondary Antibodies

 

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Additional HIF-2 alpha/EPAS1 Products

Blogs on HIF-2 alpha/EPAS1.

Check out the latest blog posts on HIF-2 alpha/EPAS1.
Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.
HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.
HIF-2 alpha, Tumor Suppression and Cell Survival
HIF-2 alpha is one subunit within the HIF-2 nuclear protein that regulates cellular responses to hypoxia (low oxygen tension conditions). Hydroxylation post-translational modifications on particular HIF residues target them for degradation. Luo, et al...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol EPAS1
Uniprot