HIF-2 alpha/EPAS1 ELISA Kits: Novus Biologicals

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1 Results for "hif-2-alpha-epas1" in ELISA Kits

DYC2997-2

Human/Rat Total HIF-2 alpha/EPAS1 DuoSet IC ELISA, 2 Plate

(1 Publication)

1 Kit (for 2 Plates)

DYC2997-2

ELISA HIF-2 alpha/EPAS1 [Biotin]

Lysate prepared from HepG2 human hepatocellular carcinoma cells treated with 150 μM of CoCl2 for 18 hours were incubated in wells coated with Total HIF-2 alpha Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. The same lysate and captured proteins were electrophoresed and transferred to a PVDF membrane. The captured protein was immunoblotted with Total HIF-2 alpha Detection Antibody and the lysate was immunoblotted with anti-HIF-2 alpha antibody. Only one band corresponding to HIF-2 alpha was detected in the captured material.Cross-reactivity experiments were performed with this DuoSet IC ELISA to further determine specificity. Recombinant mouse HIF-2 alpha was assayed at 80 ng/mL and read 16.4 ng/mL (20.5% cross-reactivity). Recombinant human (rh) HIF-1 alpha and rhARNT were assayed at 80 ng/mL and did not cross-react or interfere in the assay.

Lysate prepared from 786-O human renal adenocarcinoma cells, Caki-2 human kidney clear carcinoma cells, and MCF-7 human breast cancer cells untreated or treated with 150 μM of CoCl2 for 18 hours were analyzed with both this DuoSet IC ELISA and the Human/Mouse Total HIF-1 alpha DuoSet IC ELISA (R&D Systems, Catalog # DYC1935). The 786-O cells are positive for HIF-2 alpha only while Caki-2 and MCF-7 treated with CoCl2 are positive for HIF-1 alpha only. Only the HIF-2 alpha positive 786-O cells were detected with the Human/Rat Total HIF-2 alpha /EPAS1 DuoSet IC ELISA, indicating that this DuoSet IC ELISA is specific for HIF-2 alpha .

Lysates prepared from 786-O human renal adenocarcinoma cell and HepG2 human hepatocellular carcinoma cells untreated or treated with 150 μM of CoCl2 for 18 hours were quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with anti-HIF-2 alpha antibody. The DuoSet IC ELISA results correlated well with the relative amounts of HIF-2 alpha detected by Western blot.

Lysates prepared from PC-12 rat adrenal pheochromocytoma cells untreated or treated with 150 μM of CoCl2 for 18 hours were quantified with this DuoSet IC ELISA. The same lysates were also immunoblotted (inset) with anti-HIF-2 alpha antibody (R&D Systems, Catalog # AF2886). The DuoSet IC ELISA results correlate well with the relative amounts of HIF-2 alpha detected by Western blot.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC2997-5

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	DYC2997-5

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