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GR/NR3C1 Antibody (1006903) [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U-87 MG human glioblastoma/astrocytoma cell line, A549 human lung carcinoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GR/NR3C1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U-87 MG human glioblastoma/astrocytoma cell line, A549 human lung carcinoma cell line, and C6 rat glioma cell line. PVDF membrane was ...read more
GR/NR3C1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) at 8 µg/mL for 3 hours at room temperature. Cells ...read more
GR/NR3C1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Rat GR/NR3C1 Monoclonal Antibody (Catalog # MAB10144) at 5 µg/mL for 1 hour at room temperature followed by ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GR/NR3C1 at approximately 97 kDa (as indicated) using 20 µg/mL ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clone
1006903
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB10144-SP
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GR/NR3C1 Antibody (1006903) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GR/NR3C1
Val271-Lys777
Accession # P04150
Specificity
Detects human GR/NR3C1 in direct ELISAs. Detects human and rat GR/NR3C1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB10144 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GR/NR3C1 Antibody (1006903) [Unconjugated]

  • GCR
  • glucocorticoid receptor
  • GR
  • GRGCCR
  • GRLNuclear receptor subfamily 3 group C member 1
  • NR3C1
  • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
  • nuclear receptor subfamily 3, group C, member 1

Background

GR is a glucocorticoid receptor that is expressed in almost every cell in the body and regulates genes controlling development, metabolism and the immune response. The unbound receptor resides in the cytosol of the cell, but upon binding to glucocorticoids, it is translocated to the nucleus where it regulates gene transcription. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or acts to repress the expression of pro-inflammatory proteins in the cytosol. It is also involved in chromatin remodeling and plays a role in rapid mRNA degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for GR/NR3C1 Antibody (MAB10144) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB10144:
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ICC
(1)
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(1)
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  4
reviewed by:
Patricia Carulla Martí
 ICC Human 06/19/2023
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Summary

ApplicationImmunocytochemistry
Sample TestedCultured Human Keratinocytes
SpeciesHuman
LotCMFA0119091

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Immunocytochemistry GR/NR3C1 MAB10144
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 Patricia Carulla Martí
06/19/2023
Application: ICC
Species: Human
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