![Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of Phospho-Glycogen synthase 1 on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Glycogen synthase 1 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 84 kDa Observed band size: 84 kDa Blocking and diluting buffer: 5% BSA.Exposure time: 1 minute 34 seconds](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Western-Blot-NBP2-67315-img0008.jpg "Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of Phospho-Glycogen synthase 1 on HepG2 cell lysates. Lane 1: HepG2 cells, whole cell lysate, 10ug/lane Lane 2: HepG2 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Glycogen synthase 1 antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 84 kDa Observed band size: 84 kDa Blocking and diluting buffer: 5% BSA.Exposure time: 1 minute 34 seconds")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | SR46-06 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser641 of Human Glycogen Synthase. (SwissProt: P13807 Human; SwissProt: Q9Z1E4 Mouse; Entrez Gene: 690987 Rat) |
| Modification | p Ser641 |
| Localization | Cytoplasm |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GYS1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | IP: Use at an assay dependent concentration. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
![Glycogen Synthase [p Ser641] Antibody (SR46-06)](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Glycogen Synthase [p Ser641] Antibody (SR46-06)")
Secondary Antibodies |
Isotype Controls |
Research Areas for Glycogen Synthase Antibody (NBP2-67315)Find related products by research area.
|
|
AKT Antibody Assays: A Complex Area with an Easy Solution We at Novus Biologicals place a lot of emphasis on the kinase signaling pathways. Kinases, or phosphotransferase enzymes play a key role in phosphorylation signaling. Over 500 human protein kinases have so far been discovered. They play essential role... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GYS1 |
![Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunocytochemistry-Immunofluorescence-NBP2-67315-img0002.jpg "Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Glycogen synthase [p Ser641] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0010.jpg "Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Glycogen synthase [p Ser641] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of Phospho-Glycogen synthase 1(S641) on different lysates using anti-Phospho-Glycogen synthase 1(S641) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver lysate, untreated Lane 3: Mouse liver lysate, treated with AP](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Western-Blot-NBP2-67315-img0006.jpg "Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of Phospho-Glycogen synthase 1(S641) on different lysates using anti-Phospho-Glycogen synthase 1(S641) antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver lysate, untreated Lane 3: Mouse liver lysate, treated with AP")
![Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Western blot analysis of Glycogen Synthase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. G](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Western-Blot-NBP2-67315-img0007.jpg "Western Blot: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Western blot analysis of Glycogen Synthase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. G")
![Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunocytochemistry-Immunofluorescence-NBP2-67315-img0001.jpg "Immunocytochemistry/Immunofluorescence: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Staining Phospho-Glycogen synthase 1(S641) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0003.jpg "Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0004.jpg "Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0005.jpg "Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Phospho-Glycogen synthase 1(S641) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Glycogen-Synthase-p-Ser641-Antibody-SR46-06-Immunohistochemistry-Paraffin-NBP2-67315-img0009.jpg "Immunohistochemistry-Paraffin: Glycogen Synthase [p Ser641] Antibody (SR46-06) [NBP2-67315] - Analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Phospho-Glycogen synthase 1(S641) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Western_Blot_5219.jpg)
![Simple Western AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Simple_Western_16678.jpg)
![Immunocytochemistry AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Immunocytochemistry__Immunofluorescence_19686.jpg)
![Western Blot AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_beta_1_AF2854_Western_Blot_5220.jpg)
![Immunohistochemistry AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2854_human-mouse-rat-ampk-beta-1-affinity-purified-polyclonal-ab-immunohistochemistry-252023124938.jpg)
![Immunohistochemistry AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2854_human-mouse-rat-ampk-beta-1-affinity-purified-polyclonal-ab-immunohistochemistry-252023125022.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Immunohistochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunohistochemistry_21186.jpg)
![Western Blot Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Western_Blot_11656.jpg)
![Immunocytochemistry Lipoprotein Lipase/LPL Antibody [Unconjugated]](https://images.novusbio.com/images2/Lipoprotein_Lipase_AF7197_Immunocytochemistry_11911.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")