![Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP2-16688-img0008.jpg "Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000")
| Reactivity | Hu, Rt, Mu, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Glycerol 3 Phosphate Dehydrogenase. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Predicted Species | Mouse (91%), Porcine (90%), Bovine (91%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GPD1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPD1 protein stained by GPD1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16688-img0011.jpg "Immunocytochemistry/Immunofluorescence: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPD1 protein stained by GPD1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Paraffin-embedded D54MG xenograft. GPD1 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16688-img0010.jpg "Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Paraffin-embedded D54MG xenograft. GPD1 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min")
![Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - RT2 xenograft, using GPD1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16688-img0006.jpg "Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - RT2 xenograft, using GPD1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Paraffin-embedded Mahlarvu xenograft. GPD1 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16688-img0009.jpg "Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Paraffin-embedded Mahlarvu xenograft. GPD1 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")