![Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-8S4T8-Western-Blot-NBP3-15687-img0003.jpg "Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | 8S4T8 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 450-559 of human Glycerol Kinase (P32189). IPVVKPSMPETTALGAAMAAGAAEGVGVWSLEPEDLSAVTMERFEPQINAEESEIRYSTWKKAVMKSMGWVTTQSPESGDPSIFCSLPLGFFIVSSMVMLIGARYISGIP |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GK |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 0.05% BSA, 50% glycerol, pH7.3 |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Glycerol Kinase Antibody (NBP3-15687)Find related products by research area.
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| Gene Symbol | GK |
![Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Immunohistochemistry of paraffin-embedded mouse testis using Glycerol Kinase Rabbit mAb (NBP3-15687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-8S4T8-Immunohistochemistry-Paraffin-NBP3-15687-img0002.jpg "Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Immunohistochemistry of paraffin-embedded mouse testis using Glycerol Kinase Rabbit mAb (NBP3-15687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-8S4T8-Western-Blot-NBP3-15687-img0004.jpg "Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Immunohistochemistry of paraffin-embedded rat kidney using Glycerol Kinase Rabbit mAb (NBP3-15687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-8S4T8-Immunohistochemistry-Paraffin-NBP3-15687-img0001.jpg "Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Immunohistochemistry of paraffin-embedded rat kidney using Glycerol Kinase Rabbit mAb (NBP3-15687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")