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GPD2 Antibody

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Independent Antibodies: Western Blot: GPD2 Antibody [NBP1-86121] - Staining of human colon, liver, parathyroid gland and skin using Anti-GPD2 antibody NBP1-86121 (A) shows similar protein distribution across ...read more
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP1-86121] - Staining of human parathyroid gland using Anti-GPD2 antibody NBP1-86121.
Western Blot: GPD2 Antibody [NBP1-86121] - Analysis in human cell line CACO-2.
Independent Antibodies: Western Blot: GPD2 Antibody [NBP1-86121] - Staining of human colon, liver, parathyroid gland and skin using Anti-GPD2 antibody NBP1-86121 (A) shows similar protein distribution across ...read more
Independent Antibodies: Western Blot: GPD2 Antibody [NBP1-86121] - Analysis using Anti-GPD2 antibody NBP1-86121 (A) shows similar pattern to independent antibody NBP2-38542 (B).
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP1-86121] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP1-86121] - Staining of human parathyroid gland shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: GPD2 Antibody [NBP1-86121] - Staining in human parathyroid gland and liver tissues using anti-GPD2 antibody. Corresponding GPD2 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP1-86121] - Staining of human colon.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP1-86121] - Staining of human skin.
Immunohistochemistry-Paraffin: GPD2 Antibody [NBP1-86121] - Staining of human liver using Anti-GPD2 antibody NBP1-86121.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GPD2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RRKQMNLAYVKAADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GPD2 Protein (NBP1-86121PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GPD2 Antibody

  • EC 1.1.5.3
  • GDH2
  • glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
  • GPD2
  • GPDH-M
  • GPDM
  • GPD-M
  • mGDH
  • mGPDH
  • mitochondrial glycerophosphate dehydrogenase
  • mitochondrial
  • mtGPD

Background

Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-86121
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Applications: WB, IHC

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-86121

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Bioinformatics

Gene Symbol GPD2