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Glutathione Reductase Antibody (2J5N9)

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Western Blot: Glutathione Reductase Antibody (2J5N9) [NBP3-16436] - Western blot analysis of extracts of various cell lines, using Glutathione Reductase Rabbit mAb (NBP3-16436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: Glutathione Reductase Antibody (2J5N9) [NBP3-16436] - Western blot analysis of extracts of various cell lines, using Glutathione Reductase Rabbit mAb (NBP3-16436) at 1:1000 dilution. Secondary antibody: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
2J5N9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Glutathione Reductase Antibody (2J5N9) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 423-522 of human Glutathione Reductase (P00390). TVGLTEDEAIHKYGIENVKTYSTSFTPMYHAVTKRKTKCVMKMVCANKEEKVVGIHMQGLGCDEMLQGFAVAVKMGATKADFDNTVAIHPTSSEELVTLR
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
GSR
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Glutathione Reductase Antibody (2J5N9)

  • EC 1.8.1
  • EC 1.8.1.7
  • GLUR
  • Glutathione Reductase
  • glutathione reductase, mitochondrial
  • GR
  • GRase
  • GRD1
  • GSR
  • HEL-752
  • MGC78522

Background

Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ->2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-16436
Glutathione Reductase Antibody (2J5N9)
Species: Hu, Mu, Rt
Applications: WB

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Bioinformatics

Gene Symbol GSR