![Western Blot: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Glut5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Azide-and-BSA-Free-Western-Blot-NBP2-71285-img0008.jpg "Western Blot: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Glut5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, Flow, IHC, IP, CyTOF-ready |
| Clone | OTI9F3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SLC2A5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 54.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SLC2A5 |
![Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71285-img0005.jpg "Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)")
![Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Flow cytometric Analysis of Jurkat cells, using anti-Glut5 antibody (NBP1-47980),(Red), compared to a nonspecific negative control antibody,(Blue).](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71285-img0002.jpg "Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Flow cytometric Analysis of Jurkat cells, using anti-Glut5 antibody (NBP1-47980),(Red), compared to a nonspecific negative control antibody,(Blue).")
![Western Blot: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glut5 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glut5.](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Azide-and-BSA-Free-Western-Blot-NBP2-71285-img0007.jpg "Western Blot: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glut5 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glut5.")
![Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71285-img0003.jpg "Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)")
![Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71285-img0004.jpg "Immunohistochemistry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Glut5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, NBP1-47980)")
![Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Flow cytometric Analysis of Hela cells, using anti-Glut5 antibody (NBP1-47980),(Red), compared to a nonspecific negative control antibody,(Blue).](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71285-img0001.jpg "Flow Cytometry: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Flow cytometric Analysis of Hela cells, using anti-Glut5 antibody (NBP1-47980),(Red), compared to a nonspecific negative control antibody,(Blue).")
![Immunoprecipitation: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunoprecipitation(IP) of Glut5 by using monoclonal anti-Glut5 antibodies (Negative control: IP without adding anti-Glut5 antibody). For each experiment, 500ul of DDK tagged Glut5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-Glut5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Glut5-Antibody-OTI9F3-Azide-and-BSA-Free-Immunoprecipitation-NBP2-71285-img0006.jpg "Immunoprecipitation: Glut5 Antibody (OTI9F3) - Azide and BSA Free [NBP2-71285] - Immunoprecipitation(IP) of Glut5 by using monoclonal anti-Glut5 antibodies (Negative control: IP without adding anti-Glut5 antibody). For each experiment, 500ul of DDK tagged Glut5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-Glut5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")