![Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0006.jpg "Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody and b antigen-preabsorbed antibody. Scale bar 100 um. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) and DAPI (blue) on ependymal cells. Scale bar 10 um. e-g Immunofluorescence staining of GLUT9 (magenta) and NeuN (green) showing co-localization in neurons. Scale bar 10 um. Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide made to an internal portion of human GLUT9 (within residues 500-550). [Swiss-Prot# Q9NRM0] |
| Localization | Membrane; Multi-pass membrane protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC2A9 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This GLUT9 antibody is useful for Western blot, where a band is seen ~ 55 kDa. The immunogen of this product corresponds to a cytoplasmic domain of GLUT9 protein and in immunostaining assays, cytoplasmic-membrane staining may be expected for this product. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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reviewed by:
Verified Customer |
IHC-P | Goose | 09/22/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for GLUT9 Antibody (NBP1-05054)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SLC2A9 |
![Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - GLUT9 in methacarn-fixed murine brain. Paraffin sections of methacarn-fixed murine brain were used for immunostaining. a, b Antigen absorption test. Immunohistochemistry using showing a anti-GLUT9 antibody staining in the third ventricle ependyma and parenchyma and b antigen-preabsorbed antibody. Scale bar 100 um. c GLUT9 immunoreactivity observed in brain capillaries in the cortex. Scale bar 100 um. d-f Immunofluorescence staining of d GLUT9 and e P-gp showing co-localization in capillaries. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 um.Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-NBP1-05054-img0007.jpg "Immunohistochemistry: GLUT9 Antibody [NBP1-05054] - GLUT9 in methacarn-fixed murine brain. Paraffin sections of methacarn-fixed murine brain were used for immunostaining. a, b Antigen absorption test. Immunohistochemistry using showing a anti-GLUT9 antibody staining in the third ventricle ependyma and parenchyma and b antigen-preabsorbed antibody. Scale bar 100 um. c GLUT9 immunoreactivity observed in brain capillaries in the cortex. Scale bar 100 um. d-f Immunofluorescence staining of d GLUT9 and e P-gp showing co-localization in capillaries. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 um.Image collected and cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x) licensed under a CC-BY license.")
![Western Blot: GLUT9 Antibody [NBP1-05054] - Detection of GLUT9 in human kidney membrane.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Western-Blot-NBP1-05054-img0002.jpg "Western Blot: GLUT9 Antibody [NBP1-05054] - Detection of GLUT9 in human kidney membrane.")
![Immunocytochemistry/Immunofluorescence: GLUT9 Antibody [NBP1-05054] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT9 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05054-img0005.jpg "Immunocytochemistry/Immunofluorescence: GLUT9 Antibody [NBP1-05054] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-GLUT9 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-05054] - IHC-P analysis of mouse kidney section using GLUT9 antibody at 1:200 dilution. This antibody generated an expected cytoplasmic staining in tubular cells with a more staining towards membranes in some cells but no immunopositivity in Bowmans capsules.](http://images.novusbio.com/fullsize/GLUT9-Antibody-Immunohistochemistry-Paraffin-NBP1-05054-img0004.jpg "Immunohistochemistry-Paraffin: GLUT9 Antibody [NBP1-05054] - IHC-P analysis of mouse kidney section using GLUT9 antibody at 1:200 dilution. This antibody generated an expected cytoplasmic staining in tubular cells with a more staining towards membranes in some cells but no immunopositivity in Bowmans capsules.")
![Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody & b antigen-preabsorbed antibody. Scale bar 100 µm. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) & DAPI (blue) on ependymal cells. Scale bar 10 µm. e–g Immunofluorescence staining of GLUT9 (magenta) & NeuN (green) showing co-localization in neurons. Scale bar 10 µm. D3V, dorsal third ventricle; DAPI, 4′,6-diamidino-2-phenylindole; NeuN, neuronal nucleus marker Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05054_rabbit-polyclonal-glut9-antibody-310202416171452.jpg "Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of GLUT9 in PFA-fixed murine brain sections. Frozen sections of paraformaldehyde-fixed wild-type murine brain were used for immunofluorescence staining. a, b Antigen absorption test. Immunofluorescence staining of the ependymal wall of the dorsal third ventricle using a anti-GLUT9 antibody & b antigen-preabsorbed antibody. Scale bar 100 µm. c, d Immunofluorescence staining of GLUT9 (magenta), acetylated-tubulin (Ac-Tubulin, green) & DAPI (blue) on ependymal cells. Scale bar 10 µm. e–g Immunofluorescence staining of GLUT9 (magenta) & NeuN (green) showing co-localization in neurons. Scale bar 10 µm. D3V, dorsal third ventricle; DAPI, 4′,6-diamidino-2-phenylindole; NeuN, neuronal nucleus marker Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of ABCG2 in methanol/acetone-fixed & methacarn-fixed murine brain. Fresh frozen sections of the dorsal third ventricle were prepared from wild-type (WT) & ABCG2 knockout (KO) mice & post-fixed with methanol & acetone. Immunofluorescence staining of ABCG2 was seen in choroid plexus & capillaries in sections from a WT but not in b ABCG2 KO mouse. Scale bar 100 µm. c Immunofluorescence staining of ABCG2 (magenta) & GLUT9 (green) showing ABCG2 in the choroid plexus & GLUT9 in the ependyma. Scale bar 100 µm. d–f Immunofluorescence staining of d ABCG2 & e GLUT9 using methacarn-fixed paraffin section of capillaries showing co-localization in f. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. CP, choroid plexus; D3V, dorsal third ventricle Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-05054_rabbit-polyclonal-glut9-antibody-310202416173530.jpg "Immunohistochemistry: GLUT9 Antibody - BSA Free [NBP1-05054] - Immunofluorescence staining of ABCG2 in methanol/acetone-fixed & methacarn-fixed murine brain. Fresh frozen sections of the dorsal third ventricle were prepared from wild-type (WT) & ABCG2 knockout (KO) mice & post-fixed with methanol & acetone. Immunofluorescence staining of ABCG2 was seen in choroid plexus & capillaries in sections from a WT but not in b ABCG2 KO mouse. Scale bar 100 µm. c Immunofluorescence staining of ABCG2 (magenta) & GLUT9 (green) showing ABCG2 in the choroid plexus & GLUT9 in the ependyma. Scale bar 100 µm. d–f Immunofluorescence staining of d ABCG2 & e GLUT9 using methacarn-fixed paraffin section of capillaries showing co-localization in f. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. CP, choroid plexus; D3V, dorsal third ventricle Image collected & cropped by CiteAb from the following publication (//fluidsbarrierscns.biomedcentral.com/articles/10.1186/s12987-016-0046-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
