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Glut4 Antibody (1F8) [Unconjugated]

 (17 Publications)
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Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with ...read more
Glut4 was detected in immersion fixed paraffin-embedded sections of rat heart tissue using Mouse Anti-Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) at 5 µg/mL overnight at 4 °C. Before incubation with the ...read more
Primary podocytes can be cultured from kidney organoid glomeruli. a Isolated organoid glomeruli show evidence of podocyte cell migration (OrgPods) displaying thin arborized projections (TAPs) (inset). Inverted image ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, IP, AP, ICC/IF
Clone
1F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1262-SP
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Glut4 Antibody (1F8) [Unconjugated] Summary

Immunogen
Partially purified vesicles containing Glut4
Specificity
Recognizes an epitope in the cytoplasmic portion of rat Glut4 and has been shown to bind only to the muscle-adipose isoform of the glucose transporter, which is antigenically unique from the other transport proteins (1-3). Recognizes Glut4 in human, monkey, rat, mouse, and rabbit systems. It does not recognize this protein in canine systems. Its ability to bind to glucose transporter from other species has not been tested.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC2A4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoaffinity Purification
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Immunoprecipitation
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1262 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 um filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut4 Antibody (1F8) [Unconjugated]

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Background

Glut4 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut4 is expressed in the insulin-responsive tissues, muscle and adipose tissue. Insulin stimulation leads to translocation of Glut4 from intracellular stores to the cell surface resulting in the enhancement of glucose uptake.
  1. James, et al. (1988) Nature 333:183.
  2. Fukumoto, et al. (1989) J. Biol. Chem. 264:7776.
  3. Zorzano, et al. (1989) J. Biol. Chem. 264:12358.
  4. Rodnick, et al. (1992) J. Biol. Chem. 267:6278.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut4 Antibody (MAB1262)(17)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Rabbit.

We have publications tested in 4 applications: ICC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(5)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(9)
All Applications
Filter By Species
Human
(3)
Mouse
(8)
Rat
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using MAB1262 Applications Species
Zahra Mostofinejad, Md Akheruzzaman, Md Abu Bakkar Siddik, Presheet Patkar, Nikhil V Dhurandhar, Vijay Hegde Antidiabetic E4orf1 protein prevents hepatic steatosis and reduces markers of aging-related cellular damage in high fat fed older mice BMJ Open Diabetes Research & Care 5/3/2021 [PMID: 33941552]
Tiannan Wang, Jing Wang, Xinge Hu, Xian-Ju Huang, Guo-Xun Chen Current understanding of glucose transporter 4 expression and functional mechanisms World Journal of Biological Chemistry 11/27/2020 [PMID: 33274014]
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019-03-14;9(1):4515. 2019-03-14 [PMID: 30872638] (ICC, Western Blot, Human, Rat) ICC, Western Blot Human, Rat
LJ Hale, SE Howden, B Phipson, A Lonsdale, PX Er, I Ghobrial, S Hosawi, S Wilson, KT Lawlor, S Khan, A Oshlack, C Quinlan, R Lennon, MH Little 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening Nat Commun, 2018-12-04;9(1):5167. 2018-12-04 [PMID: 30514835] (ICC, Human) ICC Human
A Tashiro, S Shibata, Y Takai, T Uchiwa, M Furuse, S Yasuo Changes in photoperiod alter Glut4 expression in skeletal muscle of C57BL/6J mice Biochem. Biophys. Res. Commun, 2017-02-09;0(0):. 2017-02-09 [PMID: 28189688] (Western Blot, Mouse) Western Blot Mouse
Famulla S, Schlich R, Sell H et al. Differentiation of human adipocytes at physiological oxygen levels results in increased adiponectin secretion and isoproterenol-stimulated lipolysis. Adipocyte. 7/1/2012 [PMID: 23700522]

Details:
Perilipin (IMG-30288). 1. WB: Unstimulated human adipocytes, Fig 4D.
Gautier E, Westerterp M, Bhagwat N, Cremers S, Shih A, Abdel-Wahab O, Lutjohann D, Randolph G, Levine R, Tall A, Yvan-Charvet L HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders. J Exp Med, 2013-01-14;210(2):339-53. 2013-01-14 [PMID: 23319699] (Western Blot, Mouse) Western Blot Mouse
Dhurandhar EJ, Dubuisson O, Mashtalir N E4orf1: a novel ligand that improves glucose disposal in cell culture. PLoS ONE, 2011-08-23;6(8):e23394. 2011-08-23 [PMID: 21886789] (Western Blot, Mouse) Western Blot Mouse
Yi Lin, Zhongjie Sun Thyroid hormone promotes insulin-induced glucose uptake by enhancing Akt phosphorylation and VAMP2 translocation in 3T3-L1 adipocytes Journal of Cellular Physiology 10/1/2011 [PMID: 21792921]
Matsuda N, Yamamoto S, Yokoo H, Tobe K, Hattori Y Nuclear factor-kappaB decoy oligodeoxynucleotides ameliorate impaired glucose tolerance and insulin resistance in mice with cecal ligation and puncture-induced sepsis. Crit. Care Med., 2009-10-01;37(10):2791-9. 2009-10-01 [PMID: 19707125] (Western Blot, Mouse) Western Blot Mouse
Show All 17 Publications.

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Bioinformatics

Gene Symbol SLC2A4