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Glut2 Antibody (199017) [Unconjugated]

Images

 
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut2 Monoclonal Antibody (Catalog # MAB1414, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed ...read more
Glut2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human Glut2 Monoclonal Antibody (Catalog # MAB1414) at 5 µg/mL overnight at 4 °C. Tissue was stained ...read more
Glut2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Mouse Anti-Human Glut2 Monoclonal Antibody (Catalog # MAB1414) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
199017
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Glut2 Antibody (199017) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Glut2
Met1-Val524
Accession # P11168
Specificity
Recognizes human Glut2 expression on human Glut2‑transfected NS0 cells, but not on control transfectants. Was shown to detect Glut2 on the surface of the human intestinal cell line Caco2 (2) in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with human Glut1 or human Glut3.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SLC2A2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB1414 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut2 Antibody (199017) [Unconjugated]

  • GLUT2 Glucose transporter type 2, liver
  • Glut2
  • GLUT-2
  • SLC2A2
  • solute carrier family 2 (facilitated glucose transporter), member 2
  • solute carrier family 2, facilitated glucose transporter member 2

Background

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney, and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1).
  1. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
  2. Mahraoui, L. et al. (1994) J. Biochem. 298:629.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut2 Antibody (MAB1414)(4)

We have publications tested in 4 confirmed species: Human, Mouse, Primate - Chlorocebus aethiops (African Green Monkey), Xenopus.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, IHC-P.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Primate - Chlorocebus aethiops (African Green Monkey)
(1)
Xenopus
(1)
All Species

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Product General Protocols

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FAQs for Glut2 Antibody (MAB1414) (0)

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Secondary Antibodies

 

Isotype Controls

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By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a ...  Read full blog post.

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Bioinformatics

Gene Symbol SLC2A2
Uniprot