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Glucagon Antibody (181402) [Unconjugated]

 (14 Publications)
 (1 Review)
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Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were ...read more
beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
181402
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC1249U-100UG
Alexa Fluor 405
IC1249V-100UG
Alexa Fluor 488
IC1249G-100UG
Alexa Fluor 532
FAB1249X
Alexa Fluor 594
IC1249T-100UG
Alexa Fluor 647
IC1249R-100UG
Alexa Fluor 700
IC1249N-100UG
Alexa Fluor 750
IC1249S-100UG
Allophycocyanin
FAB1249A
Biotin
FAB1249B
CoraFluor 1
FAB1249CL1
DyLight 350
FAB1249D
DyLight 405
FAB1249E
DyLight 488
FAB1249K
DyLight 550
FAB1249L
DyLight 594
FAB1249M
DyLight 650
FAB1249W
DyLight 680
FAB1249Y
DyLight 755
FAB1249Z
FITC
FAB1249F
HRP
FAB1249H
Janelia Fluor 525
FAB1249JF525
Janelia Fluor 549
FAB1249I
Janelia Fluor 585
FAB1249JF585
Janelia Fluor 635
FAB1249JF635
Janelia Fluor 646
FAB1249J
Janelia Fluor 669
FAB1249JF669
NL557
NL1249R
PE
FAB1249P
PerCP
FAB1249C
mFluor Violet 450 SE
FAB1249MFV450
mFluor Violet 500 SE
FAB1249MFV500
mFluor Violet 610 SE
FAB1249MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
NL1249R
Carrier Free
MAB1249-SP
Carrier Free
IC1249V-100UG
Carrier Free
IC1249U-100UG
Carrier Free
IC1249T-100UG
Carrier Free
IC1249S-100UG
Carrier Free
IC1249R-100UG
Carrier Free
IC1249N-100UG
Carrier Free
IC1249G-100UG
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Glucagon Antibody (181402) [Unconjugated] Summary

Immunogen
Human Glucagon synthetic peptide
Specificity
Detects human and mouse Glucagon.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GCG
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 3
using
MAB1249 in the following applications:
Publications
Read Publications using
MAB1249 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucagon Antibody (181402) [Unconjugated]

  • GCG
  • glicentin-related polypeptide
  • GLP1
  • GLP-1
  • Glucagon
  • glucagon-like peptide 1
  • glucagon-like peptide 2
  • GRPP

Background

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glucagon Antibody (MAB1249)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-P, Immunofluorescence, Neutralization.


Filter By Application
Flow Cytometry
(3)
ICC
(1)
IHC
(3)
IHC-P
(1)
Immunofluorescence
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB1249 Applications Species
Asadi, F;Gunawardana, SC;Dolle, RE;Piston, DW; An orally available compound suppresses glucagon hypersecretion and normalizes hyperglycemia in type 1 diabetes JCI insight 2024-01-23 [PMID: 38258903] (Immunofluorescence, Human) Immunofluorescence Human
Lyu, X;Rowley, MJ;Kulik, MJ;Dalton, S;Corces, VG; Regulation of CTCF loop formation during pancreatic cell differentiation Nature communications 2023-10-09 [PMID: 37813869] (Flow Cytometry, Human) Flow Cytometry Human
M Kohata, J Imai, T Izumi, J Yamamoto, Y Kawana, A Endo, H Sugawara, J Seiko, H Kubo, H Kawamura, T Sato, S Osaka, Y Munakata, Y Asai, S Kodama, K Takahashi, K Kaneko, H Katagiri Roles of FoxM1-driven basal beta-cell proliferation in maintenance of beta-cell mass and glucose tolerance during adulthood Journal of diabetes investigation, 2022-07-15;0(0):. 2022-07-15 [PMID: 35633298] (IHC, Mouse) IHC Mouse
Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model Cell Reports, 2022-04-12;39(2):110670. 2022-04-12 [PMID: 35417708] (Neutralization, Mouse) Neutralization Mouse
Laura Nigi, Noemi Brusco, Giuseppina E. Grieco, Daniela Fignani, Giada Licata, Caterina Formichi et al. Increased Expression of Viral Sensor MDA5 in Pancreatic Islets and in Hormone-Negative Endocrine Cells in Recent Onset Type 1 Diabetic Donors Frontiers in Immunology 3/11/2022 [PMID: 35359976]
XX Yu, WL Qiu, L Yang, YC Wang, MY He, D Wang, Y Zhang, LC Li, J Zhang, Y Wang, CR Xu Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans Cell Research, 2021-03-10;0(0):. 2021-03-10 [PMID: 33692492] (IHC, Mouse) IHC Mouse
Laura Nigi, Noemi Brusco, Giuseppina E. Grieco, Giada Licata, Lars Krogvold, Lorella Marselli et al. Pancreatic Alpha-Cells Contribute Together With Beta-Cells to CXCL10 Expression in Type 1 Diabetes Front Endocrinol (Lausanne) 9/15/2020 [PMID: 33042009]
P Rattanaamn, YM Roshorm, C Wilasrusme, N Proprom, B Ongphiphad, SC Talchai Direct suppression of human islet dedifferentiation, progenitor genes, but not epithelial to mesenchymal transition by liraglutide Heliyon, 2020-09-15;6(9):e04951. 2020-09-15 [PMID: 32995630] (Flow Cytometry, Human) Flow Cytometry Human
F Vecchio, N Lo Buono, A Stabilini, L Nigi, MJ Dufort, S Geyer, PM Rancoita, F Cugnata, A Mandelli, A Valle, P Leete, F Mancarella, PS Linsley, L Krogvold, KC Herold, HE Larsson, SJ Richardson, NG Morgan, K Dahl-Jørge, G Sebastiani, F Dotta, E Bosi, M Battaglia Abnormal neutrophil signature in the blood and pancreas of presymptomatic and symptomatic type 1 diabetes JCI Insight, 2018-09-20;3(18):. 2018-09-20 [PMID: 30232284] (IHC-P, Human) IHC-P Human
Gomez D, O'Driscoll M, Sheets T, Hruban R, Oberholzer J, McGarrigle J, Shamblott M Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. PLoS ONE, 2015-08-19;10(8):e0133862. 2015-08-19 [PMID: 26288179] (IHC, Human) IHC Human
Show All 14 Publications.

Review for Glucagon Antibody (MAB1249) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB1249:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
 IHC-P Mouse 02/04/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22069255
SpeciesMouse

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Bioinformatics

Gene Symbol GCG