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Glut1 Antibody (202915) [Alexa Fluor® 488]

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HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1418G, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
202915
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

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Glut1 Antibody (202915) [Alexa Fluor® 488] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571
Specificity
Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SLC2A1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.
Publications
Read Publications using
FAB1418G in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution Instructions


Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut1 Antibody (202915) [Alexa Fluor® 488]

  • Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis)
  • CSE
  • DYT17
  • DYT18
  • DYT9
  • EIG12
  • Glucose transporter type 1, erythrocyte/brain
  • Glut1
  • GLUT-1
  • GLUT1DS
  • HepG2 glucose transporter
  • HTLVR
  • Human T-Cell Leukemia Virus (I and II) Receptor
  • MGC141895
  • MGC141896
  • PED
  • SLC2A1
  • solute carrier family 2 (facilitated glucose transporter), member 1
  • Solute Carrier Family 2 Member 1
  • solute carrier family 2, facilitated glucose transporter member 1

Background

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

  1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
  2. Meuckler, M. et al. 1985, Science 229:941.
  3. Jones, K.S. et al. 2006, J. Virol. 8291.
  4. Takenouchi, N. et al. 2007, J. Virol. 1506.
  5. Kinet, S. et al. 2007, Retrovirology 4:31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut1 Antibody (FAB1418G)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Immunocytochemistry.


Filter By Application
IHC
(1)
Immunocytochemistry
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using FAB1418G Applications Species
Brown, JA;Faley, SL;Judge, M;Ward, P;Ihrie, RA;Carson, R;Armstrong, L;Sahin, M;Wikswo, JP;Ess, KC;Neely, MD; Rescue of impaired blood-brain barrier in tuberous sclerosis complex patient derived neurovascular unit Journal of neurodevelopmental disorders 2024-05-23 [PMID: 38783199] (Immunocytochemistry, Human) Immunocytochemistry Human
Sureshchandra S, Doratt BM, True H et al. Multimodal profiling of term human decidua demonstrates immune adaptations with pregravid obesity Cell reports 7/25/2023 [PMID: 37432849]

Details:
Used AF700 conjugation
H Kim, K Leng, J Park, AG Sorets, S Kim, A Shostak, RJ Embalabala, K Mlouk, KA Katdare, IVL Rose, SM Sturgeon, EH Neal, Y Ao, S Wang, MV Sofroniew, JM Brunger, DG McMahon, MS Schrag, M Kampmann, ES Lippmann Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin Nature Communications, 2022-11-02;13(1):6581. 2022-11-02 [PMID: 36323693] (IHC, Mouse) IHC Mouse
Clémence Martin, Théo Dhôte, Maha Zohra Ladjemi, Muriel Andrieu, Souganya Many, Vaarany Karunanithy et al. Specific circulating neutrophils subsets are present in clinically stable adults with cystic fibrosis and are further modulated by pulmonary exacerbations Frontiers in Immunology 9/29/2022 [PMID: 36248793]
Abdullah Al Mamun, Hisaki Hayashi, Aya Yamamura, Md Junayed Nayeem, Motohiko Sato Hypoxia induces the translocation of glucose transporter 1 to the plasma membrane in vascular endothelial cells The Journal of Physiological Sciences 12/1/2020 [PMID: 32962633]

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Secondary Antibodies

 

Isotype Controls

Additional Glut1 Products

Blogs on Glut1.

Deficiency of GluT1 leads to neurological problems while excess is involved in cancers
By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a ...  Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ...  Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve...  Read full blog post.

HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.

Glucose Transporter 1 (GLUT1): a Key Metabolic Neuronal Player
Glucose is the principal fuel source for the brain and GLUT1 is the only vehicle by which glucose enters the brain. In case of GLUT1 deficiency, the risk of clinical manifestations is increased in infancy and childhood, when the brain glucose demand i...  Read full blog post.

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Bioinformatics

Gene Symbol SLC2A1
Uniprot