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Ghrelin/Obestatin Antibody (45) - BSA Free

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ELISA: Ghrelin/Obestatin Antibody (45) - BSA Free [NBP1-47183] - The calibration curve of a sandwich assay for Ghrelin using NBP1-47183 as the capture antibody and NBP1-47182B as the biotinylated detection antibody.
ELISA: Ghrelin/Obestatin Antibody (45) - BSA Free [NBP1-47183] - The calibration curve of a sandwich assay for Ghrelin using NBP1-47183 as the capture antibody and NBP1-47182B as the biotinylated detection antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
45
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-47183AF350
Alexa Fluor 405
NBP1-47183AF405
Alexa Fluor 488
NBP1-47183AF488
Alexa Fluor 532
NBP1-47183AF532
Alexa Fluor 594
NBP1-47183AF594
Alexa Fluor 647
NBP1-47183AF647
Alexa Fluor 700
NBP1-47183AF700
Alexa Fluor 750
NBP1-47183AF750
Biotin
NBP1-47183B
DyLight 350
NBP1-47183UV
DyLight 405
NBP1-47183V
DyLight 488
NBP1-47183G
DyLight 550
NBP1-47183R
DyLight 594
NBP1-47183DL594
DyLight 650
NBP1-47183C
DyLight 680
NBP1-47183FR
DyLight 755
NBP1-47183IR
FITC
NBP1-47183F
HRP
NBP1-47183H
Janelia Fluor 549
NBP1-47183JF549
Janelia Fluor 646
NBP1-47183JF646
PerCP
NBP1-47183PCP
mFluor Violet 450 SE
NBP1-47183MFV450
mFluor Violet 500 SE
NBP1-47183MFV500
mFluor Violet 610 SE
NBP1-47183MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-47183-0.025mg
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Ghrelin/Obestatin Antibody (45) - BSA Free Summary

Immunogen
Synthetic human Ser3-octanoyl ghrelin-28
Epitope
Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with rat ghrelin
Specificity
Binds human octanoylated ghrelin and human des-acyl ghrelin.
Isotype
IgG1 Lambda
Clonality
Monoclonal
Host
Mouse
Gene
GHRL
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:4000
  • Western Blot
Application Notes
ELISA: NBP1-47183 binds human ghrelin in solution. NBP1-47183 (as a capture antibody) forms a pair with NBP1-47182 as a biotinylated detection antibody for measuring total human ghrelin by sandwich ELISA. WB: NBP1-47183 can be used in Western blotting.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Ghrelin/Obestatin Antibody (45) - BSA Free

  • appetite-regulating hormone
  • ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • Ghrelin/Obestatin
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons1,2. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form."Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Ghrelin/Obestatin
Antibody (45) - BSA F ...
NBP1-47183
Ghrelin/Obestatin Antibody (45) - BSA Free
Species: Hu
Applications: WB, ELISA

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Bioinformatics

Gene Symbol GHRL