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Ghrelin/Obestatin Antibody (2F4)

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Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - GHRL monoclonal antibody (M01), clone 2F4. Analysis of GHRL expression in HeLa.
Western Blot: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - GHRL monoclonal antibody (M01), clone 2F4. Analysis of GHRL expression in HeLa.
Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - Analysis of monoclonal antibody to GHRL on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.
ELISA: Ghrelin/Obestatin Antibody (2F4) [H00051738-M01] - Detection limit for recombinant GST tagged GHRL is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
2F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Ghrelin/Obestatin Antibody (2F4) Summary

Immunogen
GHRL (AAH25791.1, 1 a.a. ~ 117 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDEMEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK
Specificity
GHRL (2F4)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GHRL
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using
H00051738-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ghrelin/Obestatin Antibody (2F4)

  • appetite-regulating hormone
  • ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • Ghrelin/Obestatin
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Ghrelin/Obestatin
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H00051738-M01
Ghrelin/Obestatin Antibody (2F4)
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Applications: WB, ELISA, IHC

Publications for Ghrelin/Obestatin Antibody (H00051738-M01)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol GHRL
Entrez
OMIM
Uniprot