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Human Ghrelin ELISA Kit (Colorimetric)

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ELISA: Human Ghrelin ELISA Kit (Colorimetric) [NBP2-66709] - Samples were spiked with high concentrations of Human Ghrelin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Ghrelin ELISA Kit (Colorimetric) [NBP2-66709] - Samples were spiked with high concentrations of Human Ghrelin and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Human Ghrelin ELISA Kit (Colorimetric) [NBP2-66709] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

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Human Ghrelin ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes the acylated version of Human GHRL in samples. No significant cross-reactivity or interference between Human GHRL and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.08%
Intra-Assay
CV% < 5.78%
Spike Recovery
83-100%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GHRL

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-66709.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Ghrelin ELISA Kit (Colorimetric)

  • appetite-regulating hormone
  • Ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • GHRL
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related Peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human Ghrelin
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NBP2-66709
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Publications for Ghrelin ELISA Kit (NBP2-66709)(1)

We have publications tested in 1 application: ELISA.


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Blogs on Ghrelin.

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Ghrelin: Targeting the Hunger Hormone to Combat Obesity and Type 2 Diabetes
As the hormone ghrelin is linked to appetite and weight gain, as well as impaired glucose-induced insulin secretion, there is considerable interest in this peptide as a potential drug target. Although the overall lack of success in this field has been...  Read full blog post.
Shades of Ghrelin in Weight Homeostasis
The neuropeptide and gut hormone Ghrelin is an endogenous ligand for the growth hormone (GH)-secretagogue receptor (GHSR) within the central nervous system. This pathway has received a great deal of attention and heavy study within the last decade bec...  Read full blog post.
Appetite and Energy: A Ghrelin Balancing Act
Ghrelin is the only potent orexigenic peptide in circulation. It stimulates food intake and leads to metabolism regulation, positive energy balance, adipogenesis, and body weight gain. However, in studies using ghrelin antibodies, the physiological si...  Read full blog post.

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Contact Information

Product PDFs

  SDS
  Human Ghrelin ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol GHRL