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GATA-3 Antibody (CL8660)

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Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human skin and pancreas tissues using NBP2-88917 antibody. Corresponding GATA3 RNA-seq data are presented ...read more
Western Blot: GATA-3 Antibody (CL8660) [NBP2-88917] - Analysis in human cell line TD47D.
Immunocytochemistry/ Immunofluorescence: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of MCF7 cells using the Anti-GATA3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and ...read more
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human kidney shows strong nuclear positivity in a small subset of cells in glomeruli.
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: GATA-3 Antibody (CL8660) [NBP2-88917] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL8660
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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GATA-3 Antibody (CL8660) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to amino acids: DQPRWVSHHHPAVLNGQHPDTHHP
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GATA3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for GATA-3 Antibody (CL8660)

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3

Background

The GATA3 gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains twoGATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelialcell biology. Defects in th

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GATA-3 Antibody
(CL8660)
NBP2-88917
GATA-3 Antibody (CL8660)
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Applications: WB, ICC/IF, IHC

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GATA3