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T-bet/TBX21 Antibody (525803) [Unconjugated]

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Jurkat human acute T cell leukemia cell line was stained with Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with ice-cold methanol.
Jurkat human acute T cell leukemia cell line was stained with Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line . PVDF membrane was probed with 2 µg/mL of Human T-bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) ...read more
CD45RO+/CD8+ PBMC lymphocytes were stained with either (A) Mouse Anti-Human T‑bet/TBX21 Monoclonal Antibody (Catalog # MAB5385) or (B) isotype control antibody (Catalog # MAB002) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
525803
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC5385U-100UG
Alexa Fluor 405
IC5385V-100UG
Alexa Fluor 488
IC5385G-100UG
Alexa Fluor 532
FAB5385X
Alexa Fluor 594
IC5385T-100UG
Alexa Fluor 647
IC5385R-100UG
Alexa Fluor 700
IC5385N-100UG
Alexa Fluor 750
IC5385S-100UG
Allophycocyanin
FAB5385A
Biotin
FAB5385B
CoraFluor 1
FAB5385CL1
DyLight 350
FAB5385D
DyLight 405
FAB5385E
DyLight 488
FAB5385K
DyLight 550
FAB5385L
DyLight 594
FAB5385M
DyLight 650
FAB5385W
DyLight 680
FAB5385Y
DyLight 755
FAB5385Z
Fluorescein
IC5385F-025
Fluorescein
IC5385F
HRP
FAB5385H
Janelia Fluor 525
FAB5385JF525
Janelia Fluor 549
FAB5385I
Janelia Fluor 585
FAB5385JF585
Janelia Fluor 635
FAB5385JF635
Janelia Fluor 646
FAB5385J
Janelia Fluor 669
FAB5385JF669
PE
FAB5385P
PerCP (Peridinin-chlorophyll Protein Complex)
IC5385C-025
PerCP (Peridinin-chlorophyll Protein Complex)
IC5385C
mFluor Violet 450 SE
FAB5385MFV450
mFluor Violet 500 SE
FAB5385MFV500
mFluor Violet 610 SE
FAB5385MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC5385C
Carrier Free
IC5385F
Carrier Free
MAB5385-SP
Carrier Free
IC5385C-025
Carrier Free
IC5385F-025
Carrier Free
IC5385V-100UG
Carrier Free
IC5385U-100UG
Carrier Free
IC5385T-100UG
Carrier Free
IC5385S-100UG
Carrier Free
IC5385R-100UG
Carrier Free
IC5385N-100UG
Carrier Free
IC5385G-100UG
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T-bet/TBX21 Antibody (525803) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human T-bet
Glu326-Asn535
Accession # Q9UL17
Specificity
Detects human T-bet.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBX21
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB5385 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for T-bet/TBX21 Antibody (525803) [Unconjugated]

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

T-box expressed in T cells (T-bet), also known as T-box transcription factor TBX21, is a 62 kDa member of the T-box family of transcription factors and the Tbr1 subfamily. Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma , and initiates Th1 lineage development from naïve Th precursor cells by both activating Th1 genetic programs and by repressing the opposing Th2 programs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for T-bet/TBX21 Antibody (MAB5385)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, IHC, IHC-P.


Filter By Application
Flow Cytometry
(1)
IHC
(1)
IHC-P
(1)
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Human
(3)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for T-bet/TBX21 Antibody (MAB5385). (Showing 1 - 1 of 1 FAQs).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TBX21
Uniprot