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GATA-3 Antibody (634919) [Unconjugated]

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Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26052) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for GATA-3 full length (FL) at approximately 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GATA-3 Monoclonal Antibody (Catalog # MAB26052) followed by HRP-conjugated Anti-Mouse IgG ...read more
Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th2 cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P) and either (A) Mouse Anti-Human GATA-3 Monoclonal ...read more
MCF-7 cells were stained with Mouse Anti-Human GATA‑3 Monoclonal Antibody (Catalog # MAB26052, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clone
634919
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB26052U
Alexa Fluor 350
IC26052U-100UG
Alexa Fluor 405
IC26052V-100UG
Alexa Fluor 488
IC26052G-100UG
Alexa Fluor 532
FAB26052X
Alexa Fluor 594
IC63301T-025
Alexa Fluor 647
IC26052R-100UG
Alexa Fluor 700
IC26052N-100UG
Alexa Fluor 750
IC26052S-100UG
Allophycocyanin
FAB26052A
Allophycocyanin
IC63301A-025
Biotin
FAB26052B
DyLight 350
FAB26052D
DyLight 405
FAB26052E
DyLight 488
FAB26052K
DyLight 550
FAB26052L
DyLight 594
FAB26052M
DyLight 650
FAB26052W
DyLight 680
FAB26052Y
DyLight 755
FAB26052Z
FITC
FAB26052F
HRP
FAB26052H
Janelia Fluor 549
FAB26052I
Janelia Fluor 646
FAB26052J
PE
FAB26052P
PerCP
FAB26052C
mFluor Violet 450 SE
FAB26052MFV450
mFluor Violet 500 SE
FAB26052MFV500
mFluor Violet 610 SE
FAB26052MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC63301A-025
Carrier Free
IC63301T-025
Carrier Free
MAB26052-SP
Carrier Free
IC26052V-100UG
Carrier Free
IC26052U-100UG
Carrier Free
IC26052S-100UG
Carrier Free
IC26052R-100UG
Carrier Free
IC26052N-100UG
Carrier Free
IC26052G-100UG
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GATA-3 Antibody (634919) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Specificity
Detects human GATA-3 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-3 Antibody (634919) [Unconjugated]

  • 131320
  • GATA binding protein 3
  • GATA3
  • GATA-3
  • GATA-binding factor 3
  • GATA-binding protein 3
  • HDRMGC5199
  • MGC2346
  • MGC5445
  • trans-acting T-cell-specific transcription factor GATA-3

Background

GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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