GATA-3 Antibody (634919) [Allophycocyanin] Summary
Immunogen |
E. coli-derived recombinant human GATA-3 Pro135-Ser258 Accession # P23771 |
Specificity |
Detects human GATA-3 in flow cytometry. |
Source |
N/A |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
GATA3 |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
|
Packaging, Storage & Formulations
Storage |
Protect from light. Do not freeze.- 12 months from date of receipt, 2 to 8 °C as supplied.
|
Buffer |
Supplied in a saline solution containing BSA and Sodium Azide. |
Preservative |
Sodium Azide |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for GATA-3 Antibody (634919) [Allophycocyanin]
Background
GATA-3 is a 54-60 kDa member of the GATA family of
transcription regulating factors. There
are currently six mammalian members, each of which binds to a G-A-T-A motif
found in gene promoters. Although GATA-3
is traditionally described as being a hematopoietic transcriptional regulator,
it has been found in multiple cell types, both embryonic and adult. Postnatal cells reported to express GATA-3
include NK cells, ILCs, NKT cells, B cells, thymocytes (DN, CD4 SP, and CD8 SP)
and T cells, plus keratinocytes, sympathetic neurons, renal distal convoluted
tubule and mammary duct epithelium.
Human GATA-3 is 443 amino acids (aa) in length. It contains two GAGA-type Zn finger domains
(aa 263-287 and 317-341) and multiple phosphorylation sites. GATAs as a group are known to either activate,
or repress, gene expression, maintain transcriptional activity, and regulate
gene expression levels. Within this
framework, GATA-3 is best known to drive naïve CD4+ T cells into a Th2
phenotype, induce Th2 proliferation, and inhibit Th1 cell development via T-bet
repression. Other effects attributed to
GATA-33 include the promotion of Th9 and Treg formation, and the inhibition of
Th1, TH17 and B cell development. Over
aa 135-258, human and mouse GATA-3 share 94% aa sequence identity.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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