![Western Blot: FXR/NR1H4 Antibody [NB400-153] - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FXR (NB400-153) in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Western-Blot-NB400-153-img0006.jpg "Western Blot: FXR/NR1H4 Antibody [NB400-153] - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FXR (NB400-153) in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | A synthetic peptide made to the C-terminus of the human FXR protein. [UniProt# Q96RI1] |
| Localization | Cytoplasmic, nuclear |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NR1H4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 66 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine, 0.15M NaCl |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 12/18/2023 |
Summary
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reviewed by:
Verified Customer |
ICC | Human | 04/04/2020 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 12/27/2019 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 02/19/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for FXR/NR1H4 Antibody (NB400-153)Find related products by research area.
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| Verified Customer 12/18/2023 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 04/04/2020 |
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| Application: | ICC | |
| Species: | Human |
| Verified Customer 12/27/2019 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | NR1H4 |
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - A549 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Immunocytochemistry-Immunofluorescence-NB400-153-img0005.jpg "Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - A549 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: FXR Antibody [NB400-153] - Detection of FXR in HeLa nuclear extract using NB400-153. ECL 30 minute detection.](http://images.novusbio.com/fullsize/FXR-Antibody-Western-Blot-NB400-153-img0002.jpg "Western Blot: FXR Antibody [NB400-153] - Detection of FXR in HeLa nuclear extract using NB400-153. ECL 30 minute detection.")
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Immunocytochemistry-Immunofluorescence-NB400-153-img0004.jpg "Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
