![Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0006.jpg "Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI1C1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 300-573 of human SPG7(NP_003110) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SPG7 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in IHC reported in scientific literature (PMID 28131770). |
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| Theoretical MW | 88.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA, 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
WB | Mouse | 11/22/2020 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for SPG7 Antibody (NBP2-01860)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Immunocytochemistry-Immunofluorescence-NBP2-01860-img0005.jpg "Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.")
![Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Flow-Cytometry-NBP2-01860-img0002.jpg "Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Loss of AFG3L2 and/or YME1L is associated with elevated OMA1, diminished SPG7, and a markedly altered pattern of OPA1 protein forms. Whole-cell lysates were resolved using SDS-PAGE and western blotted with antibody to Drp1, Oma1, and Spg7. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/12/3930), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0007.jpg "Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Loss of AFG3L2 and/or YME1L is associated with elevated OMA1, diminished SPG7, and a markedly altered pattern of OPA1 protein forms. Whole-cell lysates were resolved using SDS-PAGE and western blotted with antibody to Drp1, Oma1, and Spg7. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/12/3930), licensed under a CC-BY license.")
![Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0003.jpg "Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.")
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Immunocytochemistry-Immunofluorescence-NBP2-01860-img0004.jpg "Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.")
![Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Flow-Cytometry-NBP2-01860-img0001.jpg "Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
