![Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Western-Blot-NB100-56582-img0018.jpg "Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/mL.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow |
| Clone | 3G3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXP3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||||
| Application Notes | The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||||
| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
||||
| Control |
|
||||
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Flow Cytometry GITR/TNFRSF18 Antibody (110416) [Unconjugated]](https://images.novusbio.com/images/antibody/mab689_human-gitr-tnfrsf18-mab-clone-110416-flow-cytometry-286202414437..jpg)
| Publications using NB100-56582 | Applications | Species |
|---|---|---|
| Wells JC, Smith Ann Davenport, V Morse R. The influence of regulatory T cells and mobilisation regimens on graft versus malignancy, graft versus host disease and relapse in haematopoietic progenitor cell transplantation (DBMS, University of the West of England).. Thesis. 2015-05-06 | ||
| Gavin MA, Torgerson TR, Houston E et al. Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006-04-25 [PMID: 16617117] |
Secondary Antibodies |
Isotype Controls |
Research Areas for FoxP3 Antibody (NB100-56582)Find related products by research area.
|
|
Meeting Report: 2nd International Antibody Validation Meeting Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde... Read full blog post. |
|
FOXP3 Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function. Distinctively, ... Read full blog post. |
|
FOXP3 is a Master Regulator of T Regulatory (Treg) Cells FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ... Read full blog post. |
|
FOXP3: Master Regulatory Transcriptional Factor FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody. 1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0010.jpg "Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody. 1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate.")
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody, 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0013.jpg "Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and this antibody, 5 ug/10^6 cells. Products used: anti-CD4 PE conjugate, anti-CD25 APC conjugate, anti-FOXP3 FITC conjugate.")
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of 2 x 10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0012.jpg "Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of 2 x 10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.")
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti-CD25 APC conjugate.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0017.jpg "Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the azide free version of NB100-56582. Staining of human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti-CD25 APC conjugate.")
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.1 ug/10^6 cells. Anti-CD25 FITC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0011.jpg "Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.1 ug/10^6 cells. Anti-CD25 FITC conjugate this antibody, anti-FOXP3 FITC.")
![Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.](http://images.novusbio.com/fullsize/FoxP3-Antibody-3G3-Flow-Cytometry-NB100-56582-img0014.jpg "Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control (left) and (right) at 0.5 ug/10^6 cells. Anti-CD25 APC conjugate this antibody, anti-FOXP3 FITC.")
![N/A CD25/IL-2R alpha [HRP]](https://images.novusbio.com/images2/IL-2_R_alpha_DR2A00_ELISA_167.jpg)
![N/A CD25/IL-2R alpha [HRP]](https://images.novusbio.com/images2/DATA_CD25_DR2A00_ELISA_800.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![N/A IL-17/IL-17A [Biotin]](https://images.novusbio.com/images2/DATA_IL17_DY421_ELISA_2448.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Agonist Activity CD28 Antibody (37407) [Unconjugated]](https://images.novusbio.com/images2/CD28_MAB342_Functional_Assay_2393.jpg)
![Immunocytochemistry CD28 Antibody (37407) [Unconjugated]](https://images.novusbio.com/images2/mab342_human-cd28-mab-clone-37407-immunocytochemistry-31102022134634.jpg)
![Western Blot T-bet/TBX21 Antibody (525803) [Unconjugated]](https://images.novusbio.com/images2/T-bet_MAB5385_Western_Blot_6233.jpg)
![Intracellular Staining by Flow Cytometry T-bet/TBX21 Antibody (525803) [Unconjugated]](https://images.novusbio.com/images2/T-bet_MAB5385_Flow_Cytometry_8529.jpg)
![Intracellular Staining by Flow Cytometry T-bet/TBX21 Antibody (525803) [Unconjugated]](https://images.novusbio.com/images/antibody/mab5385_human-t-bet-mab-clone-525803-intracellular-staining-by-flow-cytometry-2932024154136.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")