![Western Blot: FOXP3 Overexpression Lysate (Adult Normal) [NBL1-10817] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXP3.](http://images.novusbio.com/fullsize/FOXP3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10817-img0002.jpg "Western Blot: FOXP3 Overexpression Lysate (Adult Normal) [NBL1-10817] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXP3.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | FoxP3 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC217580 Accession#: NM_014009 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | FOXP3 |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
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| Theoretical MW | 47.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
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| Publication using NBL1-10817 | Applications | Species |
|---|---|---|
| Um S, Lee SH, Kim H et al. The Regulation of FOXP3 Expression by the Treatment of TGF-beta and the Modification of DNA Methylation in Lung Cancer Cell Line Tuberc Respir Dis. 2011-03-01 (WB, Human) | WB | Human |
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FOXP3 Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function. Distinctively, ... Read full blog post. |
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FOXP3 is a Master Regulator of T Regulatory (Treg) Cells FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ... Read full blog post. |
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FOXP3: Master Regulatory Transcriptional Factor FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom... Read full blog post. |
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| Gene Symbol | FOXP3 |
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![Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](https://images.novusbio.com/images/FoxP3-Antibody-Flow-Cytometry-NB100-39002-img0021.jpg "Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")