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Fibronectin Antibody (P1H11) [Unconjugated]

 (16 Publications)
 (2 Reviews)
 
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) ...read more
Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining; left panel) and Daudi human Burkitt's lymphoma cell line (negative staining; right panel) using Mouse ...read more
Fibronectin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 230 kDa (as indicated) using 10 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP, ICC/IF
Clone
P1H11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
IC1918A
Fluorescein
IC1918F
Phycoerythrin
IC1918P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC1918A
Carrier Free
IC1918F
Carrier Free
IC1918P
Carrier Free
MAB1918-SP
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Fibronectin Antibody (P1H11) [Unconjugated] Summary

Immunogen
Human plasma-derived Fibronectin
Specificity
Detects human Fibronectin in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 2 Reviews rated 4
using
MAB1918 in the following applications:
Publications
Read Publications using
MAB1918 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibronectin Antibody (P1H11) [Unconjugated]

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibronectin Antibody (MAB1918)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(2)
IHC
(3)
IHC-P
(1)
Neutralization
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using MAB1918 Applications Species
S Summer, E Rossmanith, M Pasztorek, C Fiedler, M Gröger, S Rauscher, V Weber, MB Fischer Mesenchymal stem cells support human vascular endothelial cells to form vascular sprouts in human platelet lysate-based matrices PLoS ONE, 2022-12-15;17(12):e0278895. 2022-12-15 [PMID: 36520838] (IHC, Human) IHC Human
J Koch, D Mönch, A Maa beta , A Mangold, M Gužvi?, T Mürdter, T Leibold, MH Dahlke, P Renner Pharmacologic Targeting of MMP2/9 Decreases Peritoneal Metastasis Formation of Colorectal Cancer in a Human Ex Vivo Peritoneum Culture Model Cancers, 2022-08-02;14(15):. 2022-08-02 [PMID: 35954423] (IHC, Human) IHC Human
T Sakai, YY Choo, O Sato, R Ikebe, A Jeffers, S Idell, T Tucker, M Ikebe Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells International Journal of Molecular Sciences, 2022-04-27;23(9):. 2022-04-27 [PMID: 35563212] (IHC, Western Blot, Human, Mouse) IHC, Western Blot Human, Mouse
Xiaoding Hu, Emilly S. Villodre, Richard Larson, Omar M. Rahal, Xiaoping Wang, Yun Gong et al. Decorin-mediated suppression of tumorigenesis, invasion, and metastasis in inflammatory breast cancer Communications Biology 1/15/2021 [PMID: 33452400]
Julia Alanen, Heidi Appelblom, Teemu Korpimaki, Heikki Kouru, Mikko Sairanen, Mika Gissler et al. Glycosylated fibronectin as a first trimester marker for gestational diabetes Archives of Gynecology and Obstetrics 10/1/2020 [PMID: 32653948]
Song-yi Jung, Poonam Malhotra, Kiana C. Nguyen, David Salzman, Yue Qi, Ethan H. Pak et al. The KRAS-variant and its impact on normal breast epithelial cell biology Cell Death & Differentiation 12/1/2019 [PMID: 30932013]
Chiang KC, Huang ST, Wu RC et al. Interferon alpha-inducible protein 27 is an oncogene and highly expressed in cholangiocarcinoma patients with poor survival Cancer Manag Res 2/28/2019 [PMID: 30881116]
Peng Li, Xufang Lang, Shungang Xia Elevated expression of microRNA‑378 in children with asthma aggravates airway remodeling by promoting the proliferation and apoptosis resistance of airway smooth muscle cells Experimental and Therapeutic Medicine 12/28/2018 [PMID: 30783418]
DJ Kim, JM Kang, SH Park, HK Kwon, SJ Song, H Moon, SM Kim, JW Seo, YH Lee, YG Kim, JY Moon, SY Lee, Y Son, SH Lee Diabetes Aggravates Post-ischaemic Renal Fibrosis through Persistent Activation of TGF-?1 and Shh Signalling Sci Rep, 2017-12-01;7(1):16782. 2017-12-01 [PMID: 29196746] (Western Blot, Human) Western Blot Human
Juan Li, Qianying Pan, Patrick D. Rowan, Timothy N. Trotter, Deniz Peker, Kellie M. Regal et al. Heparanase promotes myeloma progression by inducing mesenchymal features and motility of myeloma cells Oncotarget 3/8/2016 [PMID: 26849235]
Show All 16 Publications.

Reviews for Fibronectin Antibody (MAB1918) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB1918:
Filter by Applications
WB
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 WB Human 02/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22321604
SpeciesHuman
  4
reviewed by:
Verified Customer
 ELISA Human 02/12/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 22225866
SpeciesHuman

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0
 
 Verified Customer
02/12/2015
Application: WB
Species: Human
read this review
 
 Verified Customer
02/12/2015
Application: ELISA
Species: Human
read this review

Bioinformatics

Gene Symbol FN1