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Fibronectin Antibody (P1H11) [Phycoerythrin]

 (3 Publications)
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HepG2 human liver cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (IC002, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
HepG2 human liver cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (IC002, open histogram). To facilitate ...read more
WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin PE-conjugated Monoclonal Antibody (Catalog # IC1918P, filled histogram) or isotype control antibody (Catalog # IC002P, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
P1H11
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
IC1918A
Fluorescein
IC1918F
Unconjugated
MAB1918
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1918
Carrier Free
MAB1918
Carrier Free
IC1918A
Carrier Free
IC1918F
Carrier Free
MAB1918-SP
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Fibronectin Antibody (P1H11) [Phycoerythrin] Summary

Immunogen
Human plasma-derived Fibronectin
Specificity
Detects human Fibronectin in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC1918P in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibronectin Antibody (P1H11) [Phycoerythrin]

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibronectin Antibody (IC1918P)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining; left panel) and Daudi human Burkitt's lymphoma cell line (negative staining; right panel) using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Bioinformatics

Gene Symbol FN1