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FGF-17 Antibody (121630)

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 (1 Review)
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FGF‑17 was detected in immersion fixed paraffin-embedded sections of human kidney using Human FGF‑17 Monoclonal Antibody (Catalog # MAB319) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the basement membrane supporting epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
FGF‑17 was detected in immersion fixed paraffin-embedded sections of human kidney using Human FGF‑17 Monoclonal Antibody (Catalog # MAB319) at 8 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
121630
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB319-SP
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FGF-17 Antibody (121630) Summary

Immunogen
E. coli-derived recombinant human FGF-17
Thr23-Thr216
Accession # O60258
Specificity
Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISA, approximately 7% cross-reactivity with recombinant human (rh) FGF-5 is observed and no cross-reactivity with rhFGF-acidic, rhFGF-basic, rhFGF-4, rhFGF-6, rhFGF-7, rhFGF8b, rhFGF-9, rhFGF-10, rhFGF-17, rhFGF-18, rhFGF-19, recombinant mouse (rm) FGF-8b, rmFGF-8c and rmFGF-15 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FGF17
Purity Statement
Protein A or G purified from ascites
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB319 in the following applications:
Publications
Read Publications using
MAB319 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-17 Antibody (121630)

  • FGF-13
  • FGF17
  • FGF-17
  • fibroblast growth factor 17

Background

Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30‑70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF‑17 shares 98.6% amino acid (aa) sequence identity with mouse FGF‑17. Rat FGF‑17 shares 100% aa sequence identity with mouse FGF‑17.

  1. Hoshikawa, M. et al. (1998) Biochem. Biophys. Res. Commun. 244:187.
  2. Xu, J. et al. (1999) Mech. Dev. 83:165.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF-17 Antibody (MAB319)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(1)
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Review for FGF-17 Antibody (MAB319) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB319:
Filter by Applications
IHC-P
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 IHC-P Human 06/28/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedKidney tissue
SpeciesHuman
Lot121630

Comments

CommentsIncubated kidney sample at 8 ug/mL overnight at 4C

Product General Protocols

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Immunohistochemistry-Paraffin FGF-17 MAB319
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 Verified Customer
06/28/2022
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol FGF17
Uniprot